Human Predicted to work with:
Recombinant fragment: PAQVFGQPPC PLSQPVLLSL IQQLASDLGT RTDLKLSYLE EAVMHLDHSD PITRDHMGSV MAQVRQKLFQ FLQAEPHNSL GKAARRLSLM LHGLVTPSLP, corresponding to C terminal amino acids 1302 - 1401 of Human EDC4 with a proprietary tag (NP_055144).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. Place sample in 1X citrate buffer (pH 6.0) in pressure cooker under 125 °C for 4min and under 90 °C for 45min, cool sample subsequently.
FunctionIn the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).
Sequence similaritiesBelongs to the WD repeat EDC4 family. Contains 4 WD repeats.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.