Overview

  • Product name
  • Description
    Rabbit polyclonal to EDC4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 903-1181 of Human EDC4 (UniProt: Q6P2E9).

  • Positive control
    • NT2D1, IMR32, U-87 MG, MCF7 and Mouse brain whole cell lysates; HeLa cells; Human U87 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 152 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).
  • Sequence similarities
    Belongs to the WD repeat EDC4 family.
    Contains 4 WD repeats.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm > P-body. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • autoantigen antibody
    • Autoantigen Ge 1 antibody
    • Autoantigen Ge-1 antibody
    • Autoantigen Ge1 antibody
    • Autoantigen RCD 8 antibody
    • Autoantigen RCD-8 antibody
    • Autoantigen RCD8 antibody
    • edc4 antibody
    • EDC4_HUMAN antibody
    • Enhancer of mRNA decapping 4 antibody
    • Enhancer of mRNA-decapping protein 4 antibody
    • Ge 1 antibody
    • Hedls antibody
    • Human enhancer of decapping large subunit antibody
    • RCD 8 antibody
    see all

Images

  • Anti-EDC4 antibody (ab154026) at 1/3000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size: 152 kDa



    5% SDS PAGE
  • Anti-EDC4 antibody (ab154026) at 1/2000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 152 kDa



    5% SDS PAGE
  • Confocal Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling EDC4 with ab154026 at 1/500 dilution (Green). Right image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human U87 xenograft tissue labeling EDC4 with ab154026 at 1/100 dilution.

References

ab154026 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154026.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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