The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Application notesIs unsuitable for WB.
FunctionIn the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).
Sequence similaritiesBelongs to the WD repeat EDC4 family. Contains 4 WD repeats.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Human enhancer of decapping large subunit antibody
RCD 8 antibody
Anti-EDC4 antibody images
Immunoprecipitation - EDC4 antibody (ab72410)
Immunoprecipitation/ Western Blot of EDC4.
Lane 1: ab72410 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
Another antibody to EDC4 was used at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.
References for Anti-EDC4 antibody (ab72410)
has not yet been referenced specifically in any publications.
Publishing research using ab72410? Please let us know so that we can cite the reference in this datasheet.
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