Overview

  • Product nameAnti-EDD antibody
    See all EDD primary antibodies
  • Description
    Goat polyclonal to EDD
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Synthetic peptide: LAIKTKNFGFV, corresponding to C terminal amino acids 2789-2799 of Human EDD.

  • General notesGenBank Accession Number – NP_056986. LocusLink ID - 51366 (human); 70790 (mouse); 117060 (rat). Gene Ontology terms - ubiquitin conjugating enzyme activity; ubiquitin-protein ligase activity; ubiquitin cycle; cell proliferation; protein modification; soluble fraction; nucleus; ligase activity.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab4376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 309 kDa (predicted molecular weight: 309 kDa).Can be blocked with Human EDD peptide (ab23029).
IP Use at an assay dependent dilution.
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. No results could not be obtained after antigen retrieval at pH6.
ICC/IF Use at an assay dependent concentration. PubMed: 21148289

Target

  • FunctionE3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May be involved in maturation and/or transcriptional regulation of mRNA. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development.
  • Tissue specificityWidely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 1 PABC domain.
    Contains 1 UBR-type zinc finger.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4432411E13Rik antibody
    • AW549941 antibody
    • C77315 antibody
    • D030042K14 antibody
    • DD5 antibody
    • E3 identified by differential display antibody
    • E3 ubiquitin protein ligase, HECT domain containing, 1 antibody
    • E3 ubiquitin-protein ligase antibody
    • E3 ubiquitin-protein ligase UBR5 antibody
    • EDD 1 antibody
    • EDD antibody
    • EDD1 antibody
    • FLJ11310 antibody
    • HECT domain-containing 1 antibody
    • hHYD antibody
    • HYD antibody
    • Hyperplastic discs protein homolog antibody
    • Hyperplastic discs, Drosophila, homolog of antibody
    • KIAA0896 antibody
    • MGC57263 antibody
    • mKIAA0896 antibody
    • Progestin induced protein antibody
    • Progestin-induced protein antibody
    • Rat100 antibody
    • Ubiquitin protein ligase antibody
    • Ubiquitin protein ligase E3 component n recognin 5 antibody
    • UBR5 antibody
    • UBR5_HUMAN antibody
    see all

Anti-EDD antibody images

  • Anti-EDD antibody (ab4376) at 1/500 dilution + MDA-MB-468 breast cancer cells (25µg)

    Secondary
    Donkey anti-goat conjugated to HRP at 1/4000 dilution

    Performed under reducing conditions.

    Predicted band size : 309 kDa


    Exposure time : 5 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab4376 (4µg/ml) staining of paraffin embedded Human Testis shows nuclear staining . Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not be obtained after antigen retrieval at pH6.

References for Anti-EDD antibody (ab4376)

This product has been referenced in:
  • Hay-Koren A  et al. The EDD E3 ubiquitin ligase ubiquitinates and up-regulates beta-catenin. Mol Biol Cell 22:399-411 (2011). WB ; Human . Read more (PubMed: 21118991) »
  • Won JH  et al. Phenotypic changes in mouse pancreatic stellate cell Ca2+ signaling events following activation in culture and in a disease model of pancreatitis. Mol Biol Cell 22:421-36 (2011). ICC/IF . Read more (PubMed: 21148289) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 50 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (7% Tris-Bicine Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Sep 28 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Rat Tissue lysate - whole (lung tissue)
Total protein in input 1000 µg
Specification lung tissue
Immuno-precipitation step Protein A/G
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Submitted Oct 31 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Rabbit Tissue lysate - whole (heart tissue)
Total protein in input 1000 µg
Specification heart tissue
Immuno-precipitation step Protein A/G
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Submitted Oct 31 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Tissue lysate - whole (lung tissue)
Total protein in input 1000 µg
Specification lung tissue
Immuno-precipitation step Protein A/G
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Submitted Oct 20 2008

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rabbit Tissue lysate - whole (heart)
Loading amount 50 µg
Specification heart
Gel Running Conditions Reduced Denaturing (6% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 12 2008

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3)
Loading amount 30 µg
Specification NIH 3T3
Gel Running Conditions Reduced Denaturing (6% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 12 2008

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (C6 cell line)
Loading amount 25 µg
Specification C6 cell line
Gel Running Conditions Reduced Denaturing (4% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 04 2008

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Application Immunoprecipitation
Sample Human Cell lysate - whole cell (breast cancer cells)
Total protein in input 250 µg
Specification breast cancer cells
Immuno-precipitation step Protein A/G
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Submitted May 23 2008

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (T98G cell line)
Loading amount 25 µg
Specification T98G cell line
Gel Running Conditions Reduced Denaturing (6% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted May 02 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MDA-MB-468 breast cancer cells)
Loading amount 25 µg
Specification MDA-MB-468 breast cancer cells
Gel Running Conditions Reduced Denaturing
Blocking step SuperBlock Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Mar 12 2008

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"