Overview

  • Product nameAnti-EDF1 antibody
    See all EDF1 primary antibodies
  • Description
    Rabbit polyclonal to EDF1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (AESDWDTVTV LRKKGPTAAQ AKSKQAILAA QRRGEDVETS KKWAAGQNKQ) of Human EDF1 (NP_003783).

  • Positive control
    • THP1 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab95110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionTranscriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism.
  • Tissue specificityExpressed in brain, liver, lung, kidney and heart (at protein level). Ubiquitously expressed. More abundant in heart, pancreas, liver, intestine and adipose tissues.
  • Sequence similaritiesContains 1 HTH cro/C1-type DNA-binding domain.
  • Developmental stageExpressed in fetal tissues. More abundant in kidney.
  • DomainThe IQ motif, which is involved in calmodulin binding, overlaps with the binding domain for nuclear receptors and transcription factors. Its phosphorylation probably allows a switch between the two activities of the protein.
  • Post-translational
    modifications
    Phosphorylated (by PKA and PKC).
  • Cellular localizationCytoplasm. Nucleus. Also nuclear upon binding to NR5A1 and treatment of cells with TPA or forskolin.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAP19 antibody
    • EDF-1 antibody
    • edf1 antibody
    • EDF1_HUMAN antibody
    • Endothelial differentiation related factor 1 antibody
    • Endothelial differentiation-related factor 1 antibody
    • MBF1 antibody
    • MGC9058 antibody
    • Multiprotein bridging factor 1 antibody
    • Multiprotein-bridging factor 1 antibody
    see all

Anti-EDF1 antibody images

  • Immunofluorescence analysis of human fibroblasts fixed with methanol labelling EDF1 with ab95110 at 5ug/ml.

  • Anti-EDF1 antibody (ab95110) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP1 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 16 kDa
    Gel concentration: 10-20%

References for Anti-EDF1 antibody (ab95110)

ab95110 has not yet been referenced specifically in any publications.

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