Overview

  • Product nameAnti-EDG1 antibody
    See all EDG1 primary antibodies
  • Description
    Rabbit polyclonal to EDG1
  • Specificityab11424 detects S1P1 protein (EDG1) in transfected human cell samples. This antibody shows no cross-reactivity to S1P3.
  • Tested applicationsSuitable for: ICC/IF, WB, IP, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Cow, Cat, Monkey
  • Immunogen

    Synthetic peptide corresponding to Mouse EDG1 aa 359-372.
    Sequence:

    SHPQKDDGDNPETI


    (Peptide available as ab39763)

  • Positive control
    • Transfected HEK293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab11424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa.Can be blocked with Human EDG1 peptide (ab39763). Band represents recombinant S1P1 from transfected HEK293 cells.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 20733200

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.

Target

  • FunctionReceptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins.
  • Tissue specificityEndothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications
    S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD363 antibody
    • CHEDG 1 antibody
    • CHEDG1 antibody
    • D1S3362 antibody
    • ECGF 1 antibody
    • ECGF1 antibody
    • EDG 1 antibody
    • EDG1 antibody
    • endothelial differentiation G protein coupled receptor 1 antibody
    • Endothelial differentiation G-protein coupled receptor 1 antibody
    • Endothelial differentiation sphingolipid G protein coupled receptor 1 antibody
    • FLJ58121 antibody
    • G protein coupled sphingolipid receptor antibody
    • g protein-coupled receptor edg-1 antibody
    • S1P receptor 1 antibody
    • S1P receptor Edg 1 antibody
    • S1P receptor Edg-1 antibody
    • S1P receptor Edg1 antibody
    • S1P(1) receptor antibody
    • S1P1 antibody
    • s1pr1 antibody
    • S1PR1_HUMAN antibody
    • sphingolipid g-protein-coupled receptor 1 antibody
    • Sphingosine 1 phosphate receptor Edg 1 antibody
    • Sphingosine 1 phosphate receptor EDG1 antibody
    • sphingosine 1- phosphate receptor 1 antibody
    • Sphingosine 1-phosphate receptor 1 antibody
    • Sphingosine 1-phosphate receptor Edg-1 antibody
    • Sphingosine 1phosphate receptor type 1 S1P1 antibody
    see all

Anti-EDG1 antibody images

  • ab11424 staining EDG1 in transfected CCL39 cells by Immunocytochemistry/ Immunofluorescence.
  • ab11424 staining EDG1 in Western Blot using a transfected HEK293 cell lysate.

References for Anti-EDG1 antibody (ab11424)

This product has been referenced in:
  • Alfonso J  et al. Downregulation of Sphingosine 1-Phosphate Receptor 1 Promotes the Switch from Tangential to Radial Migration in the OB. J Neurosci 35:13659-72 (2015). IHC-FrFl ; Mouse . Read more (PubMed: 26446219) »
  • Bot M  et al. Hematopoietic sphingosine 1-phosphate lyase deficiency decreases atherosclerotic lesion development in LDL-receptor deficient mice. PLoS One 8:e63360 (2013). Flow Cyt ; Mouse . Read more (PubMed: 23700419) »

See all 7 Publications for this product

Product Wall



I am very pleased to hear you would like to accept our offer and test ab11424 in rat. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forrat and include this code in t...

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Thank you for contacting us and I am sorry to hear that ab11424 is not providing satisfactory results. I am going to refund this purchase for you. I am sorry that this antibody did not perform as stated on the datasheet. If payment has already bee...

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Thank you for making us aware that you experienced a problem with our EDG1 antibody ab11424. As my colleague mentioned, this product is guaranteed to work with mouse samples in WB and can be replaced or refunded per our Abpromise.  For our records,...

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Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (Spinal Cord)
Loading amount 80 µg
Specification Spinal Cord
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Sep 21 2011

Thank you for your inquiry. I heard back from the testing laboratory regarding the fixation/permeabilization protocol for ab11424. Aspirate culture medium and add warmed Fixation Solution (4% Formaldehyde, 37C) and incubate 15 minutes at room te...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (skeletal muscle)
Loading amount 25 µg
Specification skeletal muscle
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Username

Dr. Francesca Cencetti

Verified customer

Submitted Oct 06 2010

Thank you for your email. When ab11424 was tested in IF, transfected CCL39 cells were used. So to our knowledge it has not yet been tested in IHC. Ab13126 has only been tested for cross-reactivity with human and we don't have any information about how ...

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Thank you very much for your patience, I have just heard back from the originator of ab11424. By Western blot, this antibody detects an ~44 kDa protein representing recombinant S1P1 from transfected HEK293 cells. The figure on the website shows a Weste...

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HEK293 cells were transiently transfected with the myc-tagged receptor. After 48 hrs, cells were solubilized (in buffer containing: 1% triton X-100, 0.5% deoxycholate, 0.1% SDS.) and protein-equalized. Samples were fractionated by SDS-PAGE (12% gel)....

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The immunogen [Synthetic Peptide: S(359) H P Q K D D G D N P E T I(372)] comes from the C-terminal domain of the cytoplasmic tail. Therefore, the antibody is targeted intracellular. Not ideal for FACS, infact the cells may fall apart due to the sorti...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"