• Product name
  • Description
    Rabbit polyclonal to EDG1
  • Host species
  • Specificity
    ab11424 detects S1P1 protein (EDG1) in transfected human cell samples. This antibody shows no cross-reactivity to S1P3.
  • Tested applications
    Suitable for: ICC/IF, WB, IP, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Cow, Cat, Monkey
  • Immunogen

    Synthetic peptide corresponding to Mouse EDG1 aa 359-372.


    (Peptide available as ab39763)

  • Positive control
    • Transfected HEK293 cells.



Our Abpromise guarantee covers the use of ab11424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa.Can be blocked with Human EDG1 peptide (ab39763). Band represents recombinant S1P1 from transfected HEK293 cells.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 20733200

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.


  • Function
    Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins.
  • Tissue specificity
    Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD363 antibody
    • CHEDG 1 antibody
    • CHEDG1 antibody
    • D1S3362 antibody
    • ECGF 1 antibody
    • ECGF1 antibody
    • EDG 1 antibody
    • EDG1 antibody
    • endothelial differentiation G protein coupled receptor 1 antibody
    • Endothelial differentiation G-protein coupled receptor 1 antibody
    • Endothelial differentiation sphingolipid G protein coupled receptor 1 antibody
    • FLJ58121 antibody
    • G protein coupled sphingolipid receptor antibody
    • g protein-coupled receptor edg-1 antibody
    • S1P receptor 1 antibody
    • S1P receptor Edg 1 antibody
    • S1P receptor Edg-1 antibody
    • S1P receptor Edg1 antibody
    • S1P(1) receptor antibody
    • S1P1 antibody
    • s1pr1 antibody
    • S1PR1_HUMAN antibody
    • sphingolipid g-protein-coupled receptor 1 antibody
    • Sphingosine 1 phosphate receptor Edg 1 antibody
    • Sphingosine 1 phosphate receptor EDG1 antibody
    • sphingosine 1- phosphate receptor 1 antibody
    • Sphingosine 1-phosphate receptor 1 antibody
    • Sphingosine 1-phosphate receptor Edg-1 antibody
    • Sphingosine 1phosphate receptor type 1 S1P1 antibody
    see all


  • Lane 1 : Anti-EDG1 antibody (ab11424) at 2 µg/ml
    Lane 2 : Anti-EDG1 antibody (ab11424) at 2 mg/ml

    Lane 1 : Rat brain tissue extract
    Lane 2 : Neuro-2a Cells

    All lanes : Goat anti-rabbit IgG (H+L), HRP conjugated at 0.4 µg/ml

    Developed using the ECL technique.
  • Immunocytochemical analysis of Neuro-2a cells, labeling EDG1 with ab11424. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. Immunostaining with anti-EDG1 diluted 2µg/ml in 0.1% BSA for 3 hours at room temperature.

    Green staining - ab11424

    Blue staining - DAPI for nuclear localization 

    Red staining - F-actin

    The right-most image is a control (no primary antibody staining).

  • Flow cytometry analysis of SH-SY5Y cells, labeling EDG1 with ab11424 (3-5µg/million cells in 2.5% BSA). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes and blocked with 5% BSA for 30 minutes at room temperature.

    Red histogram - labeling with anti-EDG1 antibody

    Purple histogram - Unstained

    Green histogram - No primary antibody control

    Pink histogram - Rabbit Isotype control

  • ab11424 staining EDG1 in transfected CCL39 cells by Immunocytochemistry/ Immunofluorescence.


This product has been referenced in:
  • Hamidi Shishavan M  et al. Differential Effects of Long Term FTY720 Treatment on Endothelial versus Smooth Muscle Cell Signaling to S1P in Rat Mesenteric Arteries. PLoS One 11:e0162029 (2016). Read more (PubMed: 27583547) »
  • Alfonso J  et al. Downregulation of Sphingosine 1-Phosphate Receptor 1 Promotes the Switch from Tangential to Radial Migration in the OB. J Neurosci 35:13659-72 (2015). IHC-FrFl ; Mouse . Read more (PubMed: 26446219) »

See all 8 Publications for this product

Customer reviews and Q&As

I am very pleased to hear you would like to accept our offer and test ab11424 in rat. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forrat and include this code in t...

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Thank you for contacting us and I am sorry to hear that ab11424 is not providing satisfactory results. I am going to refund this purchase for you. I am sorry that this antibody did not perform as stated on the datasheet. If payment has already bee...

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Thank you for making us aware that you experienced a problem with our EDG1 antibody ab11424. As my colleague mentioned, this product is guaranteed to work with mouse samples in WB and can be replaced or refunded per our Abpromise.  For our records,...

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Thank you for your inquiry. I heard back from the testing laboratory regarding the fixation/permeabilization protocol for ab11424. Aspirate culture medium and add warmed Fixation Solution (4% Formaldehyde, 37C) and incubate 15 minutes at room te...

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Thank you for your email. When ab11424 was tested in IF, transfected CCL39 cells were used. So to our knowledge it has not yet been tested in IHC. Ab13126 has only been tested for cross-reactivity with human and we don't have any information about how ...

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Thank you very much for your patience, I have just heard back from the originator of ab11424. By Western blot, this antibody detects an ~44 kDa protein representing recombinant S1P1 from transfected HEK293 cells. The figure on the website shows a Weste...

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HEK293 cells were transiently transfected with the myc-tagged receptor. After 48 hrs, cells were solubilized (in buffer containing: 1% triton X-100, 0.5% deoxycholate, 0.1% SDS.) and protein-equalized. Samples were fractionated by SDS-PAGE (12% gel)....

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The immunogen [Synthetic Peptide: S(359) H P Q K D D G D N P E T I(372)] comes from the C-terminal domain of the cytoplasmic tail. Therefore, the antibody is targeted intracellular. Not ideal for FACS, infact the cells may fall apart due to the sorti...

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