Overview

  • Product nameAnti-EDG2 antibody [1G6]
    See all EDG2 primary antibodies
  • Description
    Mouse monoclonal [1G6] to EDG2
  • Tested applicationsSuitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human EDG2 (NP_001392) produced in HEK293T cell.

  • Positive control
    • HEK293T cells transfected with the pCMV6-EDG2 This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.

Properties

Applications

Our Abpromise guarantee covers the use of ab119245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 41 kDa.
Flow Cyt 1/100. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionReceptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation.
  • Tissue specificityExpressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney. Little or no expression in liver, lung, thymus, or peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell surface. Cell membrane. Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA co-localizes with RALA in the endocytic vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5031439C20 antibody
    • AI326300 antibody
    • EDG 2 antibody
    • Endothelial differentiation gene 2 antibody
    • GPCR 26 antibody
    • Gpcr26 antibody
    • Gpcr91 antibody
    • Kdt2 antibody
    • LPA 1 antibody
    • LPA receptor 1 antibody
    • LPA receptor EDG2 antibody
    • LPA-1 antibody
    • lpA1 antibody
    • Lpar1 antibody
    • LPAR1_HUMAN antibody
    • Lysophosphatidic acid receptor 1 antibody
    • Lysophosphatidic acid receptor Edg-2 antibody
    • Lysophosphatidic acid receptor EDG2 antibody
    • MGC105279 antibody
    • MGC29102 antibody
    • Mrec1.3 antibody
    • rec.1.3 antibody
    • Ventricular zone gene 1 antibody
    • vzg-1 antibody
    • VZG1 antibody
    see all

Anti-EDG2 antibody [1G6] images

  • IHC image of EDG2 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab119245, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-EDG2 antibody [1G6] (ab119245) at 1/2000 dilution

    Lane 1 : HEK293T cells transfected with the pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with the pCMV6-ENTRY EDG2 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 41 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206065)
  • HEK293T cells transfected with either EDG2 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained with 1/100 ab119245, and then analyzed by flow cytometry.

References for Anti-EDG2 antibody [1G6] (ab119245)

ab119245 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"