Overview

  • Product nameAnti-EDG2 antibody
    See all EDG2 primary antibodies
  • Description
    Rabbit polyclonal to EDG2
  • Tested applicationsSuitable for: IHC-Fr, WB, ICC, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Cow, Human
    Predicted to work with: Xenopus laevis
  • Immunogen

    Synthetic peptide:

    DRSASSLNH TILAGVHSN

    , corresponding to amino acids 342-359 of Human EDG2.

  • Positive control
    • For western blot, Mouse liver, A549 cell lysate, Rat Liver lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab23698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
WB
ICC
IHC-FoFr
  • Application notesICC: Use at a concentration of 8 µg/ml.
    IHC-Fr: Use at an assay dependent dilution (PMID 18772233).
    WB: Use at a concentration of 2 - 5 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 41 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation.
    • Tissue specificityExpressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney. Little or no expression in liver, lung, thymus, or peripheral blood leukocytes.
    • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
    • Cellular localizationCell surface. Cell membrane. Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA co-localizes with RALA in the endocytic vesicles.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5031439C20 antibody
      • AI326300 antibody
      • EDG 2 antibody
      • Endothelial differentiation gene 2 antibody
      • GPCR 26 antibody
      • Gpcr26 antibody
      • Gpcr91 antibody
      • Kdt2 antibody
      • LPA 1 antibody
      • LPA receptor 1 antibody
      • LPA receptor EDG2 antibody
      • LPA-1 antibody
      • lpA1 antibody
      • Lpar1 antibody
      • LPAR1_HUMAN antibody
      • Lysophosphatidic acid receptor 1 antibody
      • Lysophosphatidic acid receptor Edg-2 antibody
      • Lysophosphatidic acid receptor EDG2 antibody
      • MGC105279 antibody
      • MGC29102 antibody
      • Mrec1.3 antibody
      • rec.1.3 antibody
      • Ventricular zone gene 1 antibody
      • vzg-1 antibody
      • VZG1 antibody
      see all

    Anti-EDG2 antibody images

    • ab123698 staining EDG2  in Mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices, permeablized with 0.1% Triton-X, blocked with 8% serum for 60 minutes at 35°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/50 in PBS) at 4°C for 18 hours. An Alexa Fluor® 555-conjugated Goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • All lanes : Anti-EDG2 antibody (ab23698)

      Lane 1 : Solubilised Mouse Liver at 50 µg
      Lane 2 : A549 Cell Lysate at 40 µg
      Lane 3 : Rat liver lysate at 40 µg


      Predicted band size : 41 kDa

    References for Anti-EDG2 antibody (ab23698)

    This product has been referenced in:
    • Knowlden SA  et al. Regulation of T cell motility in vitro and in vivo by LPA and LPA2. PLoS One 9:e101655 (2014). WB ; Mouse . Read more (PubMed: 25003200) »
    • Ward Y  et al. CD97 amplifies LPA receptor signaling and promotes thyroid cancer progression in a mouse model. Oncogene : (2012). Mouse . Read more (PubMed: 22797060) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Brain)
    Permeabilization No
    Specification Brain
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 11 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Mouse Tissue sections (Cortical cells)
    Specification Cortical cells
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Permeabilization Yes - 0.1% Triton X
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 35°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 10 2013

    The antibody is specific for EDG2(LPAR1). We do not find any significant similarity of the peptide antigen used for LPAR1 antibody production to residues within the LPAR2 protein. The antibody should not detect EDG4 (LPAR2). I hope this is helpful. Ple...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"