Overview

  • Product nameAnti-EDG2 antibody
    See all EDG2 primary antibodies
  • Description
    Rabbit polyclonal to EDG2
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence within human EDG2.

  • Positive control
    • Human brain tissue; Jurkat cell extracts; MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab77940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionReceptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation.
  • Tissue specificityExpressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney. Little or no expression in liver, lung, thymus, or peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell surface. Cell membrane. Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA co-localizes with RALA in the endocytic vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5031439C20 antibody
    • AI326300 antibody
    • EDG 2 antibody
    • Endothelial differentiation gene 2 antibody
    • GPCR 26 antibody
    • Gpcr26 antibody
    • Gpcr91 antibody
    • Kdt2 antibody
    • LPA 1 antibody
    • LPA receptor 1 antibody
    • LPA receptor EDG2 antibody
    • LPA-1 antibody
    • lpA1 antibody
    • Lpar1 antibody
    • LPAR1_HUMAN antibody
    • Lysophosphatidic acid receptor 1 antibody
    • Lysophosphatidic acid receptor Edg-2 antibody
    • Lysophosphatidic acid receptor EDG2 antibody
    • MGC105279 antibody
    • MGC29102 antibody
    • Mrec1.3 antibody
    • rec.1.3 antibody
    • Ventricular zone gene 1 antibody
    • vzg-1 antibody
    • VZG1 antibody
    see all

Anti-EDG2 antibody images

  • ab77940 at 1/500 dilution staining EDG2 in MCF7 cells by Immunofluorescence, in the absence or presence of immunising peptide.
  • ab77940 at 1/50 dilution staining EDG2 in paraffin-embedded human brain tissue by Immunohistochemistry, in the absence or presence of immunising peptide.
  • All lanes : Anti-EDG2 antibody (ab77940) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.

References for Anti-EDG2 antibody (ab77940)

ab77940 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us and sorry for the delay in getting back to you.

I can now confirm that the image we have presented on the datasheet of the anti-EDG2 antibody (ab77940) is of staining of the paraffin-embedded human brain tissue, sp...

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Thank you very much for your interest in ab77940.

I can confirm that amino acid range for this immunogenic peptide is aa1-50. This antibody reacts with extracellular domain at N-terminal EDG2 proteins.

This immunogen shows identit...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"