• Product name
  • Description
    Rabbit polyclonal to EDG2
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Chicken, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human EDG2.

    (Peptide available as ab94879.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Human brain; Human pancreas; Human kidney; HeLa whole cell; Jurkat whole cell; A549 whole cell



Our Abpromise guarantee covers the use of ab84788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa).


  • Function
    Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation.
  • Tissue specificity
    Expressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney. Little or no expression in liver, lung, thymus, or peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell surface. Cell membrane. Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA co-localizes with RALA in the endocytic vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5031439C20 antibody
    • AI326300 antibody
    • EDG 2 antibody
    • Endothelial differentiation gene 2 antibody
    • Endothelial differentiation, lysophosphatidic acid G protein coupled receptor, 2 antibody
    • GPCR 26 antibody
    • Gpcr26 antibody
    • Gpcr91 antibody
    • GPR26 antibody
    • Kdt2 antibody
    • LPA 1 antibody
    • LPA receptor 1 antibody
    • LPA receptor EDG2 antibody
    • LPA-1 antibody
    • lpA1 antibody
    • Lpar1 antibody
    • LPAR1_HUMAN antibody
    • Lysophosphatidic acid receptor 1 antibody
    • Lysophosphatidic acid receptor Edg-2 antibody
    • Lysophosphatidic acid receptor EDG2 antibody
    • MGC105279 antibody
    • MGC29102 antibody
    • Mrec1.3 antibody
    • rec.1.3 antibody
    • Ventricular zone gene 1 antibody
    • vzg-1 antibody
    • VZG1 antibody
    see all


  • All lanes : Anti-EDG2 antibody (ab84788) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human pancreas tissue lysate - total protein (ab29816)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 6 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 1 minute

    Human Lysophosphatidic acid receptor Edg-2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • ICC/IF image of ab84788 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84788, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


ab84788 has not yet been referenced specifically in any publications.

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