The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1 - 1/1000.
Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.
Expressed in all tissues, but most abundantly in heart, placenta, kidney, and liver.
Belongs to the G-protein coupled receptor 1 family.
ab115463 at 10 µg/ml staining EDG3 in Formalin-fixed, Paraffin-embedded Human kidney tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.