Overview

  • Product name
  • Description
    Rabbit polyclonal to EDG3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human EDG3.

    (Peptide available as ab74476.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa, U-87 MG, SW480 and Caco 2

Properties

Applications

Our Abpromise guarantee covers the use of ab74477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 42 kDa).

Target

  • Function
    Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.
  • Tissue specificity
    Expressed in all tissues, but most abundantly in heart, placenta, kidney, and liver.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EDG 3 antibody
    • EDG3 antibody
    • Endothelial differentiation G protein coupled receptor 3 antibody
    • Endothelial differentiation G-protein coupled receptor 3 antibody
    • Endothelial differentiation gene 3 antibody
    • Endothelial differentiation sphingolipid G protein coupled receptor 3 antibody
    • FLJ37523 antibody
    • FLJ93220 antibody
    • G protein coupled receptor endothelial differentiation gene 3 antibody
    • LPB 3 antibody
    • LPB3 antibody
    • MGC71696 antibody
    • OTTHUMP00000215691 antibody
    • S1P receptor 3 antibody
    • S1P receptor Edg 3 antibody
    • S1P receptor Edg-3 antibody
    • S1P receptor EDG3 antibody
    • S1P3 antibody
    • S1pr3 antibody
    • S1PR3_HUMAN antibody
    • Sphingosine 1 phosphate receptor 3 antibody
    • Sphingosine 1 phosphate receptor Edg 3 antibody
    • Sphingosine 1 phosphate receptor Edg3 antibody
    • Sphingosine 1-phosphate receptor 3 antibody
    • Sphingosine 1-phosphate receptor Edg-3 antibody
    see all

Images

  • All lanes : Anti-EDG3 antibody (ab74477) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Additional bands at : 52 kDa (possible glycosylated form).

    Exposure time : 20 minutes

References

This product has been referenced in:
  • Bernhart E  et al. Interference with distinct steps of sphingolipid synthesis and signaling attenuates proliferation of U87MG glioma cells. Biochem Pharmacol 96:119-30 (2015). Read more (PubMed: 26002572) »

See 1 Publication for this product

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There are currently no Customer reviews or Questions for ab74477.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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