Recombinant
RabMAb

Anti-EDG3 antibody [EPR4541(2)] (ab126622)

Overview

  • Product name
    Anti-EDG3 antibody [EPR4541(2)]
    See all EDG3 primary antibodies
  • Description
    Rabbit monoclonal [EPR4541(2)] to EDG3
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human EDG3 (Q99500).

  • Positive control
    • Human kidney tissue; Human heart, HepG2 and 293T cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab126622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.
    • Tissue specificity
      Expressed in all tissues, but most abundantly in heart, placenta, kidney, and liver.
    • Sequence similarities
      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • EDG 3 antibody
      • EDG3 antibody
      • Endothelial differentiation G protein coupled receptor 3 antibody
      • Endothelial differentiation G-protein coupled receptor 3 antibody
      • Endothelial differentiation gene 3 antibody
      • Endothelial differentiation sphingolipid G protein coupled receptor 3 antibody
      • FLJ37523 antibody
      • FLJ93220 antibody
      • G protein coupled receptor endothelial differentiation gene 3 antibody
      • LPB 3 antibody
      • LPB3 antibody
      • MGC71696 antibody
      • OTTHUMP00000215691 antibody
      • S1P receptor 3 antibody
      • S1P receptor Edg 3 antibody
      • S1P receptor Edg-3 antibody
      • S1P receptor EDG3 antibody
      • S1P3 antibody
      • S1pr3 antibody
      • S1PR3_HUMAN antibody
      • Sphingosine 1 phosphate receptor 3 antibody
      • Sphingosine 1 phosphate receptor Edg 3 antibody
      • Sphingosine 1 phosphate receptor Edg3 antibody
      • Sphingosine 1-phosphate receptor 3 antibody
      • Sphingosine 1-phosphate receptor Edg-3 antibody
      see all

    Images

    • All lanes : Anti-EDG3 antibody [EPR4541(2)] (ab126622) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Human heart tissue lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 42 kDa
      Observed band size : 42 kDa
    • ab126622, at a 1/50 dilution, staining EDG3 in paraffin-embedded Human kidney tissue by immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126622 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab126622.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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