Overview

  • Product name
  • Description
    Rabbit polyclonal to EDG4
  • Specificity
    This antibody is specific for EDG4 (N term).
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    NETIGFFYNNSGKELSSHWRP

    conjugated to KLH, corresponding to N terminal amino acids 10/30 of Human EDG4

  • Positive control
    • Breast carcinoma tissue and HL60 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab38322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/25 - 1/100.
WB 1/100 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

Target

  • Function
    Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Plays a key role in phospholipase C-beta (PLC-beta) signaling pathway. Stimulates phospholipase C (PLC) activity in a manner that is independent of RALA activation.
  • Tissue specificity
    Expressed most abundantly in testes and peripheral blood leukocytes with less expression in pancreas, spleen, thymus and prostate. Little or no expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney, ovary, small intestine, or colon.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell surface. Cell membrane. Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA colocalizes with RALA in the endocytic vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • EDG 4 antibody
    • EDG-4 antibody
    • EDG4 antibody
    • Endothelial Cell Differentiation Gene 4 antibody
    • Endothelial differentiation lysophosphatidic acid G protein coupled receptor 4 antibody
    • FLJ93869 antibody
    • G protein coupled receptor antibody
    • IPA2 antibody
    • LPA 2 antibody
    • LPA receptor 2 antibody
    • LPA receptor EDG4 antibody
    • LPA-2 antibody
    • LPA2 antibody
    • LPAR2 antibody
    • LPAR2_HUMAN antibody
    • Lysophosphatidic acid receptor 2 antibody
    • Lysophosphatidic acid receptor Edg 4 antibody
    • Lysophosphatidic acid receptor Edg-4 antibody
    • Lysophosphatidic acid receptor EDG4 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling EDG4 with ab38322 at 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Anti-EDG4 antibody (ab38322) at 1/1000 dilution + HL-60 whole cell lysate at 35 µg/ml

    Secondary
    goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    Additional bands at : 15 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Blocking/Dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Sample
Mouse Tissue sections (Cortical)
Specification
Cortical
Permeabilization
Yes - 0.3% TritonX
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 24 2013

Thank you for contacting us.

Your credit note ID for the refund of ab38322 is xxx.
Your credit note ID for the refund of ab104803 is xxx.

I am sorry that this antibody did not perform as stated on the datasheet. If payment ha...

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Thank you very much for your interest in ab38322.

To our knowledge, ab38322 has not been tested in Flow cytometry and we do not offer samples. Therefore, I can offer a discount off a future purchase if you buy ab38322 now, test it in Flow cy...

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Merci de nous avoir contactés. Je peux confirmer que pour tester cet anticorps sur du tissue en paraffine, un démasquage au tampon Citrate pH 6 avait été utilisé. Veuillez trouver le protocole en attaché. Je peux aussi conseiller de con...

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