Overview

  • Product nameAnti-EDR1 / PHC1 antibody
    See all EDR1 / PHC1 primary antibodies
  • Description
    Mouse monoclonal to EDR1 / PHC1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: PVGCSQLLKE SEKPLQTGLP TGLTENQSGG PLGVDSPSAE LDKKANLLKC EYCGKYAPAE QFRGSKRFCS MTCAKRYNVS CSHQFRLKRK KMKEFQEANY , corresponding to amino acids 751-851 of Human EDR1/ PHC1

Properties

Applications

Our Abpromise guarantee covers the use of ab54954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
    • Sequence similaritiesContains 1 FCS-type zinc finger.
      Contains 1 SAM (sterile alpha motif) domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Early development regulatory protein 1 antibody
      • hPH1 antibody
      • mPH1 antibody
      • PH1 antibody
      • Phc1 antibody
      • PHC1_HUMAN antibody
      • Polyhomeotic-like protein 1 antibody
      • Rae28 antibody
      see all

    Anti-EDR1 / PHC1 antibody images

    • Western blot against tagged recombinant protein immunogen using ab54954 EDR1 / PHC1 antibody at 1ug/ml. Predicted band size of immunogen is 43 kDa

    References for Anti-EDR1 / PHC1 antibody (ab54954)

    ab54954 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (U2OS)
    Loading amount 25 µg
    Specification U2OS
    Gel Running Conditions Non-reduced Non-Denaturing (Native) (12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Submitted Aug 01 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"