Overview

  • Product nameAnti-EEA1 antibody
    See all EEA1 primary antibodies
  • Description
    Rabbit polyclonal to EEA1
  • Tested applicationsSuitable for: WB, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    GSQGSDLDSSATPINTVDVNNESSSEG

    conjugated to KLH by a C-terminal Cysteine residue linker, corresponding to amino acids 24 - 40 of Human EEA1

  • Positive control
    • whole extract of mouse NIH-3T3 cells; human HeLa and rat NRK cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab50313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC
ICC/IF
  • Application notesICC/IF: Use at a concentration of 5 - 10 µg/ml.
    WB: Use at a concentration of 0.4 - 0.8 µg/ml. Detects a band of approximately 160 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionBinds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
    • Sequence similaritiesContains 1 C2H2-type zinc finger.
      Contains 1 FYVE-type zinc finger.
    • DomainThe FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
    • Cellular localizationCytoplasm. Early endosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Early endosome antigen 1 antibody
      • Early endosome antigen 1, 162kD antibody
      • Early endosome associated protein antibody
      • EEA 1 antibody
      • EEA1 antibody
      • EEA1_HUMAN antibody
      • Endosome associated protein p162 antibody
      • Endosome-associated protein p162 antibody
      • MST105 antibody
      • MSTP105 antibody
      • ZFYVE2 antibody
      • Zinc finger FYVE domain containing protein 2 antibody
      • Zinc finger FYVE domain-containing protein 2 antibody
      see all

    Anti-EEA1 antibody images

    • HeLa cells fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1 µg/mL ab50313."
    • ab50313 staining EEA1 in mouse primary astrocytes by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.05% saponin and blocking with 10% serum was performed for 20 minutes at 250C. Samples were incubated with primary antibody (1/150: in 10% goat serum / PBS) for 1 hour at 25°C. An Abcam`s ab6939, Cy3®-conjugated goat polyclonal to rabbit IgG (H&L) was used as secondary antibody at 1/200 dilution.

      See Abreview

    References for Anti-EEA1 antibody (ab50313)

    This product has been referenced in:
    • Schorch B  et al. LRP1 is a receptor for Clostridium perfringens TpeL toxin indicating a two-receptor model of clostridial glycosylating toxins. Proc Natl Acad Sci U S A 111:6431-6 (2014). ICC/IF ; Mouse . Read more (PubMed: 24737893) »
    • Glauser DL  et al. Virion endocytosis is a major target for murid herpesvirus-4 neutralization. J Gen Virol 93:1316-27 (2012). Read more (PubMed: 22377583) »

    See all 6 Publications for this product

    Product Wall

    Thank you very much for your responses.
    I'm sending a free of charge vial of ab95195 on the order ***, which should arrive tomorrow. Please keep me updated about the results using this antibody.
    For the replacement of ab50313, I certainly und...

    Read More

    Thank you very much for your reply and for sending all of this information.
    I will be happy to send the replacement ab95195, but I will need the original order number or PO number that ab2074 was received on. Would you be able to provide this info...

    Read More

    Thanks for the update. We certainly appreciate your lab's troubleshooting efforts. Please feel free to reply after your trip.

    We have previously been in contact regarding ab2074, which was not providing satisfactory results. We had offered a free of charge replacement with a different antibody, but we were waiting to hear from you with your selection. If you have dete...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Mouse Cultured Cells (primary astrocyte)
    Specification primary astrocyte
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.05% saponin
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 08 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Lung carcinoma cell line)
    Specification Lung carcinoma cell line
    Fixative Paraformaldehyde
    Permeabilization Yes - Triton X-100
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 17 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"