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GSQGSDLDSSATPINTVDVNNESSSEGconjugated to KLH by a C-terminal Cysteine residue linker, corresponding to amino acids 24 - 40 of Human EEA1
Our Abpromise guarantee covers the use of ab50313 in the following tested applications.
ab50313 staining EEA1 in mouse primary astrocytes by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.05% saponin and blocking with 10% serum was performed for 20 minutes at 250C. Samples were incubated with primary antibody (1/150: in 10% goat serum / PBS) for 1 hour at 25°C. An Abcam`s ab6939, Cy3®-conjugated goat polyclonal to rabbit IgG (H&L) was used as secondary antibody at 1/200 dilution.