Overview

  • Product nameAnti-EEA1 antibody
    See all EEA1 primary antibodies
  • Description
    Rabbit polyclonal to EEA1
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Platypus (Ornithorhynchus anatinus), Elephant
  • Immunogen

    Synthetic peptide corresponding to a region within the C terminal amino acids 1361-1411 (CGKGFSVTVR RHHCRQCGNI FCAECSAKNA LTPSSKKPVR VCDACFNDLQ G) of Human EEA1, NP_003557.2

  • Positive control
    • Whole cell lysate from HeLa cells, 293T cells and NIH3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab86566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 162 kDa).
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionBinds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
    • Sequence similaritiesContains 1 C2H2-type zinc finger.
      Contains 1 FYVE-type zinc finger.
    • DomainThe FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
    • Cellular localizationCytoplasm. Early endosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Early endosome antigen 1 antibody
      • Early endosome antigen 1, 162kD antibody
      • Early endosome associated protein antibody
      • EEA 1 antibody
      • EEA1 antibody
      • EEA1_HUMAN antibody
      • Endosome associated protein p162 antibody
      • Endosome-associated protein p162 antibody
      • MST105 antibody
      • MSTP105 antibody
      • ZFYVE2 antibody
      • Zinc finger FYVE domain containing protein 2 antibody
      • Zinc finger FYVE domain-containing protein 2 antibody
      see all

    Anti-EEA1 antibody images

    • All lanes : Anti-EEA1 antibody (ab86566) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg
      Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg


      Predicted band size : 162 kDa
      Observed band size : 200 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

    References for Anti-EEA1 antibody (ab86566)

    ab86566 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"