The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
Zinc finger FYVE domain containing protein 2 antibody
Zinc finger FYVE domain-containing protein 2 antibody
Western blot - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg) Lane 2: Early Endosome Marker knockout HAP1 whole cell lysate (20 µg) Lane 3: HeLa whole cell lysate (20 µg) Lane 4: NIH3T3 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109110 observed at 162 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab109110 was shown to recognize Early Endosome Marker in wild-type HAP1 cells as signal was lost at the expected MW in Early Endosome Marker knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Early Endosome Marker knockout samples were subjected to SDS-PAGE. Ab109110 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - EEA1 antibody [EPR4245] (ab109110)
All lanes : Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110) at 1/10000 dilution
Lane 1 : COS-1 cell lysate Lane 2 : NIH 3T3 cell lysate Lane 3 : C6 cell lysate Lane 4 : HeLa cell lysate Lane 5 : Jurkat cell lysate Lane 6 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution