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Product name Anti-EEA1 antibody [EPR4245] - Early Endosome Marker See all EEA1 primary antibodies
Rabbit monoclonal [EPR4245] to EEA1 - Early Endosome Marker
Tested applications Suitable for:
WB, ICC more details Unsuitable for:
Flow Cyt,IHC-P or IP
Mouse, Rat, Human
Synthetic peptide corresponding to residues in the N terminus of Human EEA1 (UniProt Q15075).
COS-1, NIH 3T3, C6, HeLa, Jurkat, and JAR cell lysates
This product is a recombinant rabbit monoclonal antibody.
Alternative versions available:
Anti-EEA1 antibody (Alexa Fluor [EPR4245] ( ® 488) - Early Endosome Marker ab185039) Anti-EEA1 antibody (Alexa Fluor [EPR4245] ( ® 647) - Early Endosome Marker ab196186) Anti-EEA1 antibody (HRP) - Early Endosome Marker [EPR4245] ( ab195553) Anti-EEA1 antibody (Alexa Fluor[EPR4245] ( ® 594)-Early Endosome Marker ab206913)
Produced using Abcam's RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number EPR4245
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 170 kDa (predicted molecular weight: 162 kDa).
1/500 - 1/1000.
Application notes Is unsuitable for Flow Cyt,IHC-P or IP.
Function Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Sequence similarities Contains 1 C2H2-type zinc finger. Contains 1 FYVE-type zinc finger.
Domain The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
Cellular localization Cytoplasm. Early endosome membrane.
Information by UniProt
Early endosome antigen 1 antibody
Early endosome antigen 1, 162kD antibody
Early endosome associated protein antibody
Anti-EEA1 antibody [EPR4245] - Early Endosome Marker images
Western blot - EEA1 antibody [EPR4245] (ab109110)
All lanes : Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110) at 1/10000 dilution Lane 1 : COS-1 cell lysate Lane 2 : NIH 3T3 cell lysate Lane 3 : C6 cell lysate Lane 4 : HeLa cell lysate Lane 5 : Jurkat cell lysate Lane 6 : JAR cell lysate Lysates/proteins at 10 µg per lane. Secondary HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size : 162 kDa Observed band size : 170 kDa ( why is the actual band size different from the predicted?)
References for Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"