Recombinant
RabMAb

Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110)

Overview

  • Product name
    Anti-EEA1 antibody [EPR4245] - Early Endosome Marker
    See all EEA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4245] to EEA1 - Early Endosome Marker
  • Tested applications
    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the N terminus of Human EEA1 (UniProt Q15075).

  • Positive control
    • COS-1, NIH 3T3, C6, HeLa, Jurkat, and JAR cell lysates
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 170 kDa (predicted molecular weight: 162 kDa).
ICC 1/500 - 1/1000.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
    • Sequence similarities
      Contains 1 C2H2-type zinc finger.
      Contains 1 FYVE-type zinc finger.
    • Domain
      The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
    • Cellular localization
      Cytoplasm. Early endosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Early endosome antigen 1 antibody
      • Early endosome antigen 1, 162kD antibody
      • Early endosome associated protein antibody
      • EEA 1 antibody
      • EEA1 antibody
      • EEA1_HUMAN antibody
      • Endosome associated protein p162 antibody
      • Endosome-associated protein p162 antibody
      • MST105 antibody
      • MSTP105 antibody
      • ZFYVE2 antibody
      • Zinc finger FYVE domain containing protein 2 antibody
      • Zinc finger FYVE domain-containing protein 2 antibody
      see all

    Images

    • All lanes : Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (ab109110) at 1/10000 dilution

      Lane 1 : COS-1 cell lysate
      Lane 2 : NIH 3T3 cell lysate
      Lane 3 : C6 cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : Jurkat cell lysate
      Lane 6 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 162 kDa
      Observed band size : 170 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Wolff NA  et al. Evidence for mitochondrial localization of divalent metal transporter 1 (DMT1). FASEB J 28:2134-45 (2014). Read more (PubMed: 24448823) »
    • Choi S  et al. Globotriaosylceramide induces lysosomal degradation of endothelial KCa3.1 in fabry disease. Arterioscler Thromb Vasc Biol 34:81-9 (2014). Read more (PubMed: 24158513) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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