Recombinant
RabMAb

Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (Alexa Fluor® 488) (ab185039)

Overview

  • Product name
    Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (Alexa Fluor® 488)
    See all EEA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4245] to EEA1 - Early Endosome Marker (Alexa Fluor® 488)
  • Conjugation
    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human EEA1 (N terminal).
    Database link: Q15075

  • Positive control
    • Flow Cyt: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50.

ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody.

Target

  • Function
    Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
  • Sequence similarities
    Contains 1 C2H2-type zinc finger.
    Contains 1 FYVE-type zinc finger.
  • Domain
    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • Cellular localization
    Cytoplasm. Early endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Early endosome antigen 1 antibody
    • Early endosome antigen 1, 162kD antibody
    • Early endosome associated protein antibody
    • EEA 1 antibody
    • EEA1 antibody
    • EEA1_HUMAN antibody
    • Endosome associated protein p162 antibody
    • Endosome-associated protein p162 antibody
    • MST105 antibody
    • MSTP105 antibody
    • ZFYVE2 antibody
    • Zinc finger FYVE domain containing protein 2 antibody
    • Zinc finger FYVE domain-containing protein 2 antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab185039 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab185039, 1/50 dilution) for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

ab185039 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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