Overview

  • Product name
    Anti-EED antibody [163C]
    See all EED primary antibodies
  • Description
    Mouse monoclonal [163C] to EED
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
    Unsuitable for: IHC-Fr or WB
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein (His-tag) corresponding to Human EED aa 1-441.
    Database link: O75530

  • Positive control
    • Human tonsil, testicle, bone marrow, thymus and lymph node tissues; mouse lymph node tissue; V5-tagged Human EED expressed in Hek293T cells;

Properties

Applications

Our Abpromise guarantee covers the use of ab126542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/40.

Antigen retrieval (Tris-EDTA buffer) recommended.

IHC-P 1/75 - 1/600.

Antigen Retrieval: 20 min ER2 (Tris-EDTA)

  • Application notes
    Is unsuitable for IHC-Fr or WB.
  • Target

    • Function
      Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
    • Tissue specificity
      Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer.
    • Sequence similarities
      Belongs to the WD repeat ESC family.
      Contains 7 WD repeats.
    • Developmental stage
      Expression peaks at the G1/S phase boundary.
    • Cellular localization
      Nucleus. Chromosome. Transiently colocalizes with XIST at inactive X chromosomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • eed antibody
      • EED protein antibody
      • EED, mouse, homolog of antibody
      • EED_HUMAN antibody
      • Embryonic ectoderm development antibody
      • Embryonic ectoderm development isoform a antibody
      • Embryonic ECTODERM development protein homolog antibody
      • Embryonic ectoderm development protein, mouse, homolog of antibody
      • hEED antibody
      • OTTHUMP00000235483 antibody
      • OTTHUMP00000235484 antibody
      • OTTHUMP00000235485 antibody
      • Polycomb protein eed antibody
      • WAIT 1 antibody
      • WAIT-1 antibody
      • WAIT1 antibody
      • WD protein associating with integrin cytoplasmic tails 1 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin embedded Human lymph node (top left), thymus (top right), testicle (bottom left) and bone marrow (bottom right) tissues labeling EED with ab126542 antibody at 1/600 dilution.

    • Immunohistochemical analysis of paraffin embedded mouse lymph node tissue labeling EED with ab126542 antibody at 1/75 dilution.

    • Immunohistochemical analysis of on frozen cytospins preparations of V5-tagged Human EED expressed in Hek293T labeling EED with ab126542 antibody (left). Cytospin preparation of V5-tagged Human MNDA protein was used as a negative control (middle). Labeling with an anti-V5 mAb confirmed the efficiency of transfection (right).

      .

    • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling EED with ab126542 antibody at 1/40 dilution (green). DAPI was used to counterstain the nuclei (blue).

    References

    This product has been referenced in:
    • Li J  et al. EZH2-mediated H3K27 trimethylation mediates neurodegeneration in ataxia-telangiectasia. Nat Neurosci 16:1745-53 (2013). Read more (PubMed: 24162653) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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