Overview

  • Product nameAnti-EED antibody
    See all EED primary antibodies
  • Description
    Rabbit polyclonal to EED
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Chicken, Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Xenopus laevis, Zebrafish, Xenopus tropicalis , Medaka fish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 21-70 (QKLSSDENSN PDLSGDENDD VSIESGTNTE PDTPTNTPNA GRKSWGK) of Human EED (NP_694536)

  • Positive control
    • HepG2 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab113911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 45 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionPolycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
  • Tissue specificityExpressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer.
  • Sequence similaritiesBelongs to the WD repeat ESC family.
    Contains 7 WD repeats.
  • Developmental stageExpression peaks at the G1/S phase boundary.
  • Cellular localizationNucleus. Chromosome. Transiently colocalizes with XIST at inactive X chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • eed antibody
    • EED protein antibody
    • EED, mouse, homolog of antibody
    • EED_HUMAN antibody
    • Embryonic ectoderm development antibody
    • Embryonic ectoderm development isoform a antibody
    • Embryonic ECTODERM development protein homolog antibody
    • Embryonic ectoderm development protein, mouse, homolog of antibody
    • hEED antibody
    • OTTHUMP00000235483 antibody
    • OTTHUMP00000235484 antibody
    • OTTHUMP00000235485 antibody
    • Polycomb protein eed antibody
    • WAIT 1 antibody
    • WAIT-1 antibody
    • WAIT1 antibody
    • WD protein associating with integrin cytoplasmic tails 1 antibody
    see all

Anti-EED antibody images

  • Anti-EED antibody (ab113911) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 45 kDa

References for Anti-EED antibody (ab113911)

ab113911 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Chicken Tissue lysate - whole (brain)
Loading amount 5.2 µg
Specification brain
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Ms. Ying Jiang

Verified customer

Submitted Oct 08 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"