• Product name
  • Description
    Rabbit polyclonal to EED
  • Specificity
    ab4469 specifically recognises the four isoforms of EED following the immunoprecipitation of tagged Ezh2 from HEK293 nuclear extract. However, using HEK293 nuclear lysate ab4469 predominantly recognises non-specific bands.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Cow, Human
    Predicted to work with: Mouse, Chicken, Xenopus laevis
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human EED.

    (Peptide available as ab13743.)

  • Positive control
    • This antibody gave a positive signal in Human Recombinant EED Protein



Our Abpromise guarantee covers the use of ab4469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200. Predicted molecular weight: 49 kDa.
ICC/IF 1/100.


  • Function
    Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
  • Tissue specificity
    Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer.
  • Sequence similarities
    Belongs to the WD repeat ESC family.
    Contains 7 WD repeats.
  • Developmental stage
    Expression peaks at the G1/S phase boundary.
  • Cellular localization
    Nucleus. Chromosome. Transiently colocalizes with XIST at inactive X chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • eed antibody
    • EED protein antibody
    • EED, mouse, homolog of antibody
    • EED_HUMAN antibody
    • Embryonic ectoderm development antibody
    • Embryonic ectoderm development isoform a antibody
    • Embryonic ECTODERM development protein homolog antibody
    • Embryonic ectoderm development protein, mouse, homolog of antibody
    • hEED antibody
    • OTTHUMP00000235483 antibody
    • OTTHUMP00000235484 antibody
    • OTTHUMP00000235485 antibody
    • Polycomb protein eed antibody
    • WAIT 1 antibody
    • WAIT-1 antibody
    • WAIT1 antibody
    • WD protein associating with integrin cytoplasmic tails 1 antibody
    see all

Anti-EED antibody images

  • Predicted band size : 49 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: EED knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab4469 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab4469 was shown to recognize EED when EED knockout samples were used, along with additional cross-reactive bands. Wild-type and EED knockout samples were subjected to SDS-PAGE. ab4469 and ab8245 (loading control to GAPDH) were diluted 1/250 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Rabbit polyclonal to EED (ab4469) on U20S cells. Antibody used at 1/100.

    U20S cells were fixed with PFA (3.7%), permeabilised with PBS 0.1% Triton. Counterstained with Hoechst. Cells were examined with Axioplan 2 microscope (Zeiss).

    Secondary antibody: donkey anti-rabbit (Cy3).

  • Anti-EED antibody (ab4469) at 1/250 dilution + EED Recombinant Protein (Human) at 0.1 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    ab4469 recognizes the full-length recombinant EED protein which has an expected molecular weight of 80 kDa (tagged).

References for Anti-EED antibody (ab4469)

This product has been referenced in:
  • Mitic T  et al. EZH2 modulates angiogenesis in vitro and in a mouse model of limb ischemia. Mol Ther 23:32-42 (2015). Read more (PubMed: 25189741) »
  • Gonzalez ME  et al. EZH2 expands breast stem cells through activation of NOTCH1 signaling. Proc Natl Acad Sci U S A 111:3098-103 (2014). WB ; Human . Read more (PubMed: 24516139) »

See all 13 Publications for this product

Product Wall

Western blot
Human Cell lysate - whole cell (DLD1)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 02 2017

Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (10%)
Mouse Cell lysate - whole cell (Extra Embryonic Endoderm Cells (XEN))
Extra Embryonic Endoderm Cells (XEN)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 27 2013

Thank you for contacting us. I am sorry that the results did not improve. I would like to ask how you would like to proceed with this case. In order to help you further I would need more information about order number. The order number you have...

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