Overview

  • Product name
  • Description
    Rabbit polyclonal to eEF1A1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 131-180 (GQTREHALLA YTLGVKQLIV GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK) of Rat eEF1A1 (XP_002725693.1).

  • Positive control
    • Rat liver lysate IF/ICC: HeLa cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab118703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
  • Tissue specificity
    Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle.
  • Sequence similarities
    Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCS 3 antibody
    • CCS3 antibody
    • Cervical cancer suppressor 3 antibody
    • chunp6927 antibody
    • CTCL tumor antigen antibody
    • EE1A1 antibody
    • EEF 1 antibody
    • EEF1A antibody
    • eEF1A-1 antibody
    • EEF1A1 antibody
    • EF-1-alpha-1 antibody
    • EF-Tu antibody
    • EF1A antibody
    • EF1a like protein antibody
    • EF1A1_HUMAN antibody
    • Elongation factor 1 alpha subunit antibody
    • Elongation factor 1-alpha 1 antibody
    • Elongation factor Tu antibody
    • Eukaryotic elongation factor 1 A-1 antibody
    • Eukaryotic translation elongation factor 1 alpha 1 antibody
    • Eukaryotic translation elongation factor 1 alpha 1 like 14 antibody
    • Glucocorticoid receptor AF 1 specific elongation factor antibody
    • GRAF 1EF antibody
    • HNGC:16303 antibody
    • ik:tdsubc_2a3 antibody
    • ik:tdsubc_2b3 antibody
    • LENG7 antibody
    • Leukocyte receptor cluster (LRC) member 7 antibody
    • Leukocyte receptor cluster member 7 antibody
    • Prostate tumor inducing protein 1 antibody
    • PTI1 antibody
    • tdsubc_2a3 antibody
    • Translation elongation factor 1 alpha 1 like 14 antibody
    • wu:fa91c07 antibody
    • wu:fa94b03 antibody
    • wu:fi13b09 antibody
    • xx:tdsubc_2a3 antibody
    • xx:tdsubc_2b3 antibody
    see all

Images

  • ab118703 stained HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab118703 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-eEF1A1 antibody (ab118703) at 1 µg/ml + Rat liver lysate at 10 µg

    Predicted band size: 50 kDa



    Gel concentration: 12%

References

ab118703 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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