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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNAs
Overview
Product name
Anti-eEF1A1+eEF1A2+eEF1AL3 antibody
Description
Rabbit polyclonal to eEF1A1+eEF1A2+eEF1AL3
Specificity
Due to sequence homology the antibody recognises all three eEF1A forms, eEF1A1, eEF1A2 and eEF1A3. Through WB ab37969 was found to detect recombinant and endogenous eF1A1 and eF1A2.
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Sheep, Rabbit, Horse, Chicken, Hamster, Cow, Cat, Dog, Pig, Chimpanzee, Macaque Monkey
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human eEF1A1.
(Peptide available as ab37968.)
Positive control
This antibody gave a positive signal in the following: Human Whole Cell Lysates (HeLa, Jurkat, A431, HEK 293, HepG2, MCF-7, SHSY-5Y, U2OS) Mouse Whole Cell Lysates (NIH 3T3, MEF1) Mouse Tissue Lysates (Brain, Liver, Heart, Kidney, Pancreas, Testis, Skeletal muscle, Spinal Cord, Ovary) Rat Whole Cell Lysate(PC12) Rat Tissue Lysate (Brain, Liver, Heart)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNAs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
Target
Relevance
Function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Tissue specificity eEF1A1: Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle. Tissue specificity eEF1A2: Brain, heart, and skeletal muscle. Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. PTM eEF1A1: ISGylated.
Alternative names
- CCS3 antibody
- eEF1A-1 antibody
- eEF1A-2 antibody
see all
Database links
- SwissProt: P68104 Human
- SwissProt: Q05639 Human
- SwissProt: Q5VTE0 Human
- SwissProt: P10126 Mouse
- SwissProt: P62630 Rat
Anti-eEF1A1+eEF1A2+eEF1AL3 antibody images:
Western blot - Anti-eEF1A1 antibody (ab37969)
All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 50 kDa
Immunocytochemistry/ Immunofluorescence - eEF1A1 antibody (ab37969)
ICC/IF image of ab37969 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37969, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Western blot - eEF1A1 antibody (ab37969)
All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 5 : Heart (Mouse) Tissue Lysate
Lane 6 : Kidney (Mouse) Tissue Lysate
Lane 7 : Pancreas (Mouse) Tissue Lysate (ab29363)
Lane 8 : Testis (Mouse) Tissue Lysate
Lane 9 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)
Lane 10 : Spinal Cord (Mouse) Tissue Lysate
Lane 11 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 12 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 13 : Brain (Rat) Tissue Lysate - normal tissue
Lane 14 : Liver (Rat) Tissue Lysate
Lane 15 : Heart (Rat) Tissue Lysate
Lane 16 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 50 kDa
Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-eEF1A1 antibody (ab37969)
All lanes : Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969) at 1 µg/ml
Lane 1 : eEF1A1 protein (Tagged) (ab81792)
Lane 2 : EEF1A2 protein (T7 Tag) (ab40317)
Lysates/proteins at 0.1 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 50 kDa
Exposure time : 3 minutes
References for Anti-eEF1A1+eEF1A2+eEF1AL3 antibody (ab37969)
This product has been referenced in:
- Hashimoto K & Ishima T Neurite Outgrowth Mediated by Translation Elongation Factor eEF1A1: A Target for Antiplatelet Agent Cilostazol. PLoS One 6:e17431 (2011). WB; Rat.Read more (PubMed: 21390260) »
- Vessey JPet al. Mammalian Pumilio 2 regulates dendrite morphogenesis and synaptic function. Proc Natl Acad Sci U S A 107:3222-7 (2010). WB.Read more (PubMed: 20133610) »
See all 6 publications for this product
Publishing research using ab37969? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"