Anti-EEF1A2 antibody (ab212172)
Key features and details
- Rabbit polyclonal to EEF1A2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EEF1A2 antibody
See all EEF1A2 primary antibodies -
Description
Rabbit polyclonal to EEF1A2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human EEF1A2 aa 200-300.
Database link: Q05639 -
Positive control
- WB; RT-4 and U-251 MG whole cell lysates. IHC-P: Human fallopian tube, duodenum, kidney and skeletal muscle tissues. ICC: U-2 OS whole cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 50 kDa.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 50 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. -
Tissue specificity
Brain, heart, and skeletal muscle. -
Sequence similarities
Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 515233 Cow
- Entrez Gene: 1917 Human
- Entrez Gene: 13628 Mouse
- Entrez Gene: 24799 Rat
- Omim: 602959 Human
- SwissProt: Q32PH8 Cow
- SwissProt: Q05639 Human
- SwissProt: P62631 Mouse
see all -
Alternative names
- eEF1A-2 antibody
- Eef1a2 antibody
- EEF1AL antibody
see all
Images
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Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling EEF1A2 in the cytosol with ab212172 at 2 µg/ml.
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Immunohistochemical analysis of human fallopian tube labeling EEF1A2 with ab212172 at a 1/200 dilution.
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All lanes : Anti-EEF1A2 antibody (ab212172) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate
Predicted band size: 50 kDa -
Immunohistochemical analysis of human duodenum labeling EEF1A2 with ab212172 at a 1/200 dilution.
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Immunohistochemical analysis of human kidney labeling EEF1A2 with ab212172 at a 1/200 dilution.
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Immunohistochemical analysis of human skeletal muscle labeling EEF1A2 with ab212172 at a 1/200 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab212172 has not yet been referenced specifically in any publications.