Overview

  • Product nameAnti-EEF1A2 antibody
    See all EEF1A2 primary antibodies
  • Description
    Rabbit polyclonal to EEF1A2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, Zebrafish
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Pig
  • Immunogen

    Synthetic peptide: VIDCHTAHIA CKFAELKEKI DRRSGKKLED NPKSLKSGDA AIVEMVPGKP, corresponding to a region within internal sequence amino acids 360 - 409 of Human EEF1A2 (NP_001949)

  • Positive control
    • Fetal brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab82912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/12500.

Target

Anti-EEF1A2 antibody images

  • Anti-EEF1A2 antibody (ab82912) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • All lanes : Anti-EEF1A2 antibody (ab82912) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

References for Anti-EEF1A2 antibody (ab82912)

ab82912 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"