Overview

  • Product nameAnti-eEF1B2 antibody
    See all eEF1B2 primary antibodies
  • Description
    Rabbit polyclonal to eEF1B2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Chicken, Cow, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human eEF1B2.

    (Peptide available as ab37956.)

  • Positive control
    • HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate, Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate, A431 (Human epithelial carcinoma cell line) Whole Cell Lysate, HEK 293 (Human embryonic kidney cell line) Whole Cell Lysate, HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate, MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate and U2OS (Human osteosarcoma cell line) Whole Cell Lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionEF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.
  • Sequence similaritiesBelongs to the EF-1-beta/EF-1-delta family.
    Contains 1 GST C-terminal domain.
  • Post-translational
    modifications
    Phosphorylation affects the GDP/GTP exchange rate.
  • Information by UniProt
  • Database links
  • Alternative names
    • EEF1B antibody
    • EEF1B1 antibody
    • EEF1B2 antibody
    • EF-1-beta antibody
    • EF1B antibody
    • EF1B_HUMAN antibody
    • Elongation factor 1, beta-2-A antibody
    • Elongation factor 1-beta antibody
    • eukaryotic translation elongation factor 1 beta 1 antibody
    • eukaryotic translation elongation factor 1 beta 2 antibody
    see all

Anti-eEF1B2 antibody images

  • All lanes : Anti-eEF1B2 antibody (ab37957) at 1/250 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)
    Lane 4 : HEK293 whole cell lysate (ab7902)
    Lane 5 : HepG2 whole cell lysate (ab7900)
    Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab37957 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37957, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

References for Anti-eEF1B2 antibody (ab37957)

ab37957 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"