Overview

  • Product nameAnti-eEF1B2 antibody
    See all eEF1B2 primary antibodies
  • Description
    Mouse monoclonal to eEF1B2
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow
  • Immunogen

    Recombinant fragment: VPSQADVAVF EAVSSPPPAD LCHALRWYNH IKSYEKEKAS LPGVKKALGK YGPADVEDTT GSG, corresponding to amino acids 29-91 of Human eEF1B2

  • Positive control
    • HeLa cell lysate. Recombinant tagged human eEF1B2 fragment.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 25 kDa.

Target

Anti-eEF1B2 antibody images

  • Anti-eEF1B2 antibody (ab77043) at 1 µg/ml + Recombinant tagged human eEF1B2 fragment at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 25 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Anti-eEF1B2 antibody (ab77043) at 1 µg/ml + HeLa cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 25 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

References for Anti-eEF1B2 antibody (ab77043)

ab77043 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"