Overview

  • Product name
  • Description
    Rabbit polyclonal to EEF1D
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 130 and 180 of Human EEF1D (NP_001951.2)

  • Positive control
    • HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab85964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 32 kDa.
IP Use at 2-5 - 5 µg/mg of lysate.

Target

  • Function
    Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.
    Isoform 2: Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE).
  • Tissue specificity
    Isoform 2 is specifically expressed in brain, cerebellum and testis.
  • Sequence similarities
    Belongs to the EF-1-beta/EF-1-delta family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen NY CO 4 antibody
    • Antigen NY-CO-4 antibody
    • EEF 1D antibody
    • EEF1D antibody
    • EF 1 delta antibody
    • EF 1D antibody
    • EF-1-delta antibody
    • EF1D antibody
    • EF1D_HUMAN antibody
    • Elongation factor 1 delta antibody
    • Elongation factor 1-delta antibody
    • eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) antibody
    • Eukaryotic translation elongation factor 1 delta antibody
    • FP1047 antibody
    • Guanine nucleotide exchange protein antibody
    see all

Images

  • All lanes : Anti-EEF1D antibody (ab85964) at 0.04 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg
    Lane 3 : Hela whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 32 kDa


    Exposure time : 3 seconds
  • 1mg HeLa whole cell lysate was immunoprecipitated using 3µg ab85964.
    20% of IP was loaded per lane and probed with with ab85964 at 1 µg/ml (lane 1) and with a control IgG (lane 4).
    Detection: chemiluminescence with an exposure time of 1 second.

References

ab85964 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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