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Product name Anti-EEF1G antibody [EPR7200] See all EEF1G primary antibodies
Rabbit monoclonal [EPR7200] to EEF1G
Tested applications Suitable for:
WB, IP, IHC-P, Flow Cyt, ICC/IF more details
Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human EEF1G.
293T, HL60, C6, RAW 264.7, PC12, NIH 3T3, HepG2 and HeLa cell lysates; Human pancreatic adenocarcinoma tissue; HeLa cells.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K D)
K D = 1.31 x 10
Learn more about K D
Storage buffer pH: 7.20 Preservative: 0.01% Sodium azide Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purity Tissue culture supernatant
Clone number EPR7200
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
1/10 - 1/100.
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
1/1000 - 1/10000.
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/100 - 1/250.
Function Probably plays a role in anchoring the complex to other cellular components.
Tissue specificity Highly expressed in pancreatic tumor tissue and to a lesser extent in normal kidney, intestine, pancreas, stomach, lung, brain, spleen and liver.
Sequence similarities Contains 1 EF-1-gamma C-terminal domain. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Information by UniProt
eEF 1B gamma antibody
Anti-EEF1G antibody [EPR7200] images
Flow Cytometry - Anti-EEF1G antibody [EPR7200] (ab124994)
Overlay histogram showing HeLa cells stained with ab124994 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124994, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) ( ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10 6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - Anti-EEF1G antibody [EPR7200] (ab124994)
All lanes : Anti-EEF1G antibody [EPR7200] (ab124994) at 1/10000 dilution Lane 1 : 293T cell lysate Lane 2 : HL60 cell lysate Lane 3 : C6 cell lysate Lane 4 : RAW 264.7 cell lysate Lane 5 : PC12 cell lysate Lane 6 : NIH 3T3 cell lysate Lane 7 : HepG2 cell lysate Lane 8 : HeLa cell lysate Lysates/proteins at 10 µg per lane. Secondary Goat anti-Rabbit HRP at 1/2000 dilution Predicted band size : 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF1G antibody [EPR7200] (ab124994)
ab124994 at 1/250 dilution staining EEF1G in paraffin-embedded Human pancreatic adenocarcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-EEF1G antibody [EPR7200] (ab124994)
ab124994 at 1/100 dilution staining EEF1G in HeLa cells by Immunofluorescence.
Other-Anti-EEF1G antibody [EPR7200](ab124994)
References for Anti-EEF1G antibody [EPR7200] (ab124994)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"