Overview

  • Product name
    Anti-EEF1G antibody [EPR7200]
    See all EEF1G primary antibodies
  • Description
    Rabbit monoclonal [EPR7200] to EEF1G
  • Tested applications
    Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human EEF1G.

  • Positive control
    • 293T, HL60, C6, RAW 264.7, PC12, NIH 3T3, HepG2 and HeLa cell lysates; Human pancreatic adenocarcinoma tissue; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 1.31 x 10 -11 M
    Learn more about KD
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 0.05% BSA, 50% Glycerol
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR7200
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
Flow Cyt 1/1000 - 1/10000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.

Target

  • Function
    Probably plays a role in anchoring the complex to other cellular components.
  • Tissue specificity
    Highly expressed in pancreatic tumor tissue and to a lesser extent in normal kidney, intestine, pancreas, stomach, lung, brain, spleen and liver.
  • Sequence similarities
    Contains 1 EF-1-gamma C-terminal domain.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2610301D06Rik antibody
    • AA407312 antibody
    • eEF 1B gamma antibody
    • EEF 1G antibody
    • eEF-1B gamma antibody
    • EEF1G antibody
    • EF 1 gamma antibody
    • EF 1G antibody
    • EF-1-gamma antibody
    • EF1 gamma antibody
    • EF1G antibody
    • EF1G_HUMAN antibody
    • Elongation factor 1 gamma antibody
    • Elongation factor 1-gamma antibody
    • Eukaryotic translation elongation factor 1 gamma antibody
    • GIG 35 antibody
    • GIG35 antibody
    • MGC103354 antibody
    • MGC114210 antibody
    • MGC144723 antibody
    • MGC144724 antibody
    • MGC94929 antibody
    • Pancreatic tumor related protein antibody
    • PRO1608 antibody
    • Translation elongation factor eEF 1 gamma chain antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab124994 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124994, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-EEF1G antibody [EPR7200] (ab124994) at 1/10000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HL60 cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : RAW 264.7 cell lysate
    Lane 5 : PC12 cell lysate
    Lane 6 : NIH 3T3 cell lysate
    Lane 7 : HepG2 cell lysate
    Lane 8 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size : 50 kDa
  • ab124994 at 1/250 dilution staining EEF1G in paraffin-embedded Human pancreatic adenocarcinoma tissue by Immunohistochemistry.
  • ab124994 at 1/100 dilution staining EEF1G in HeLa cells by Immunofluorescence.
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:
  • Milone MR  et al. Proteomic analysis of zoledronic-acid resistant prostate cancer cells unveils novel pathways characterizing an invasive phenotype. Oncotarget 6:5324-41 (2015). WB . Read more (PubMed: 25481874) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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