The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
1/50 - 1/100.
1/20. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
FunctionCatalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Sequence similaritiesBelongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
Post-translational modificationsPhosphorylation by EF-2 kinase completely inactivates EF-2. Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis. ISGylated.
ab40812 at a 1/100 dilution staining EEF2 in human breast carcinoma tissue by Immunohistochemistry, Paraffin embedded section.
References for Anti-EEF2 antibody (ab40812)
This product has been referenced in:
Leal G et al. Neuronal activity induces synaptic delivery of hnRNP A2/B1 by a BDNF-dependent mechanism in cultured hippocampal neurons. PLoS One9:e108175 (2014).
Read more (PubMed: 25286195) »
Sung VM & Tsai CL ADP-Ribosylargininyl reaction of cholix toxin is mediated through diffusible intermediates. BMC Biochem15:26 (2014).
Read more (PubMed: 25494717) »