• Product nameAnti-EEF2 antibody
    See all EEF2 primary antibodies
  • Description
    Rabbit polyclonal to EEF2
  • Tested applicationsSuitable for: WB, ICC, Flow Cyt, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the C-terminus of Human EEF2.

  • Positive control
    • HEK293 and Human breast carcinoma tissue.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA, 49% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab40812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
ICC 1/50 - 1/100.
Flow Cyt 1/20. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/50.
IHC-P Use at an assay dependent concentration.


  • FunctionCatalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
  • Sequence similaritiesBelongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
  • Post-translational
    Phosphorylation by EF-2 kinase completely inactivates EF-2.
    Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • EEF 2 antibody
    • Eef2 antibody
    • EF-2 antibody
    • EF2 antibody
    • EF2_HUMAN antibody
    • Elongation factor 2 antibody
    • Eukaryotic translation elongation factor 2 antibody
    • Polypeptidyl tRNA translocase antibody
    • SCA26 antibody
    see all

Anti-EEF2 antibody images

  • Anti-EEF2 antibody (ab40812) at 1/50000 dilution + 10ug HEK293

    Predicted band size : 95 kDa
    Observed band size : 95 kDa
  • ab40812 at a 1/100 dilution staining EEF2 in human breast carcinoma tissue by Immunohistochemistry, Paraffin embedded section.

References for Anti-EEF2 antibody (ab40812)

This product has been referenced in:
  • Leal G  et al. Neuronal activity induces synaptic delivery of hnRNP A2/B1 by a BDNF-dependent mechanism in cultured hippocampal neurons. PLoS One 9:e108175 (2014). WB ; Rat . Read more (PubMed: 25286195) »
  • Sung VM & Tsai CL ADP-Ribosylargininyl reaction of cholix toxin is mediated through diffusible intermediates. BMC Biochem 15:26 (2014). WB . Read more (PubMed: 25494717) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse pancreatic acinar cells)
Loading amount 30 µg
Specification Mouse pancreatic acinar cells
Gel Running Conditions Reduced Non-Denaturing (Native) (4-20 %)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

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Submitted Oct 02 2012