Overview

  • Product name
  • Description
    Rabbit polyclonal to EEF2
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Xenopus laevis, Carp, Snake, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 808 - 858 of Human EEF2 (NP_001952.1)

  • Positive control
    • Human: breast carcinoma, colon carcinoma, linitis plastica stomach cancer, ovarian carcinoma, prostate carcinoma, skin melanoma, small cell lung cancer, stomach adenocarcinoma, testicular seminoma. Mouse: colon carcinoma CT26, teratoma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500. Antigen retrieval with citrate buffer may enhance staining.
    Note: expected to work on frozen sections.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
    • Sequence similarities
      Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
    • Post-translational
      modifications
      Phosphorylation by EF-2 kinase completely inactivates EF-2.
      Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
      ISGylated.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • EEF 2 antibody
      • Eef2 antibody
      • EF-2 antibody
      • EF2 antibody
      • EF2_HUMAN antibody
      • Elongation factor 2 antibody
      • Eukaryotic translation elongation factor 2 antibody
      • Polypeptidyl tRNA translocase antibody
      • SCA26 antibody
      see all

    Images

    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human colon carcinoma, using 1/100 ab84755 in conjunction with 1/100 Goat anti-Rabbit IgG conjugated with a fluorescent dye (red).
    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human testicular seminoma, using 1/100 ab84755 followed by DAB staining.
    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Mouse teratoma, using 1/100 ab84755 followed by DAB staining.

    References

    ab84755 has not yet been referenced specifically in any publications.

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    There are currently no Customer reviews or Questions for ab84755.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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