Overview

  • Product name
    Anti-EEF2 antibody [EP723Y]
    See all EEF2 primary antibodies
  • Description
    Rabbit monoclonal [EP723Y] to EEF2
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of human eEF2 was used as immunogen.

  • Positive control
    • 293 cell lysate, human lymphoma (IHC-P).
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EP723Y
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab40762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/50.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
    • Sequence similarities
      Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
    • Post-translational
      modifications
      Phosphorylation by EF-2 kinase completely inactivates EF-2.
      Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
      ISGylated.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • EEF 2 antibody
      • Eef2 antibody
      • EF-2 antibody
      • EF2 antibody
      • EF2_HUMAN antibody
      • Elongation factor 2 antibody
      • Eukaryotic translation elongation factor 2 antibody
      • Polypeptidyl tRNA translocase antibody
      • SCA26 antibody
      see all

    Images

    • Anti-EEF2 antibody [EP723Y] (ab40762) at 1/1000 dilution + HEK293 cell lysate at 10 µg

      Predicted band size : 95 kDa
      Observed band size : 95 kDa
    • ab40762 (1/50) staining human lymphoma by immunohistochemistry, paraffin embedded tissue.

    References

    ab40762 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab40762.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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