Anti-EEF2 (phospho T56) antibody (ab115165)

Overview

  • Product nameAnti-EEF2 (phospho T56) antibody
    See all EEF2 primary antibodies
  • Description
    Rabbit polyclonal to EEF2 (phospho T56)
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region of Human EEF2 phosphorylated at Threonine 56.

  • Positive control
    • K562 cell extract; NIH3T3 cell extract

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20ºC.
  • Storage bufferpH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa). (Colorimetric).

Target

  • FunctionCatalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
  • Sequence similaritiesBelongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
  • Post-translational
    modifications
    Phosphorylation by EF-2 kinase completely inactivates EF-2.
    Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
    ISGylated.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • EEF 2 antibody
    • Eef2 antibody
    • EF-2 antibody
    • EF2 antibody
    • EF2_HUMAN antibody
    • Elongation factor 2 antibody
    • Eukaryotic translation elongation factor 2 antibody
    • Polypeptidyl tRNA translocase antibody
    • SCA26 antibody
    see all

Anti-EEF2 (phospho T56) antibody images

  • All lanes : Anti-EEF2 (phospho T56) antibody (ab115165) at 1 µg/ml

    Lane 1 : K562 cell extract
    Lane 2 : K562 cell extract treated with Anisomycin
    Lane 3 : NIH3T3 cell extract
    Lane 4 : NIH3T3 cell extract treated with Anisomycin


    Predicted band size : 95 kDa
    Observed band size : 95 kDa

References for Anti-EEF2 (phospho T56) antibody (ab115165)

ab115165 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab115165.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"