Purification notesab53114 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 24130777
1/300 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
Use at an assay dependent concentration.
FunctionCatalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Sequence similaritiesBelongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
Post-translational modificationsPhosphorylation by EF-2 kinase completely inactivates EF-2. Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis. ISGylated.
ab53114 at 1/50 dilution staining EEF2 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.ab53114 at 1/50 dilution staining EEF2 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
References for Anti-EEF2 (phospho T56) antibody (ab53114)
This product has been referenced in:
Grandjean M et al. Hypoxia Integration in the Serological Proteome Analysis Unmasks Tumor Antigens and Fosters the Identification of Anti-Phospho-eEF2 Antibodies as Potential Cancer Biomarkers. PLoS One8:e76508 (2013).
Read more (PubMed: 24130777) »
Collodoro M et al. Identification and quantification of concentration-dependent biomarkers in MCF-7/BOS cells exposed to 17ß-estradiol by 2-D DIGE and label-free proteomics. J Proteomics75:4555-69 (2012).
Read more (PubMed: 22580036) »