The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Predicted molecular weight: 95 kDa.
FunctionCatalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Sequence similaritiesBelongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
Post-translational modificationsPhosphorylation by EF-2 kinase completely inactivates EF-2. Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis. ISGylated.
Western blot - EEF2 (phospho T56 + T58) antibody [EP833Y] (ab82981)
All lanes : Anti-EEF2 (phospho T56 + T58) antibody [EP833Y] (ab82981) at 1/2000 dilution
Lane 1 : Untreated 293 cell lysate Lane 2 : Serum starved 293 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 95 kDa Observed band size : 95 kDa
References for Anti-EEF2 (phospho T56 + T58) antibody [EP833Y] (ab82981)
This product has been referenced in:
Tavares CD et al. The molecular mechanism of eukaryotic elongation factor 2 kinase activation. J Biol Chem289:23901-16 (2014).
Read more (PubMed: 25012662) »
Hamdi MM & Mutungi G Dihydrotestosterone stimulates amino acid uptake and the expression of LAT2 in mouse skeletal muscle fibres through an ERK1/2-dependent mechanism. J Physiol589:3623-40 (2011).
Read more (PubMed: 21606113) »