Anti-EEF2 (phospho T56 + T58) antibody [EP833Y] (ab82981)

Overview

  • Product nameAnti-EEF2 (phospho T56 + T58) antibody [EP833Y]
    See all EEF2 primary antibodies
  • Description
    Rabbit monoclonal [EP833Y] to EEF2 (phospho T56 + T58)
  • SpecificityThe antibody only detects eEF2 phosphorylated on Tyreonine 56/58.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Thr 56/58 of human EEF2

  • Positive control
    • 293 cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEP833Y
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 95 kDa.

Target

  • FunctionCatalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
  • Sequence similaritiesBelongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.
  • Post-translational
    modifications
    Phosphorylation by EF-2 kinase completely inactivates EF-2.
    Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
    ISGylated.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • EEF 2 antibody
    • Eef2 antibody
    • EF-2 antibody
    • EF2 antibody
    • EF2_HUMAN antibody
    • Elongation factor 2 antibody
    • Eukaryotic translation elongation factor 2 antibody
    • Polypeptidyl tRNA translocase antibody
    • SCA26 antibody
    see all

Anti-EEF2 (phospho T56 + T58) antibody [EP833Y] images

  • All lanes : Anti-EEF2 (phospho T56 + T58) antibody [EP833Y] (ab82981) at 1/2000 dilution

    Lane 1 : Untreated 293 cell lysate
    Lane 2 : Serum starved 293 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 95 kDa
    Observed band size : 95 kDa

References for Anti-EEF2 (phospho T56 + T58) antibody [EP833Y] (ab82981)

This product has been referenced in:
  • Tavares CD  et al. The molecular mechanism of eukaryotic elongation factor 2 kinase activation. J Biol Chem 289:23901-16 (2014). Human . Read more (PubMed: 25012662) »
  • Hamdi MM & Mutungi G Dihydrotestosterone stimulates amino acid uptake and the expression of LAT2 in mouse skeletal muscle fibres through an ERK1/2-dependent mechanism. J Physiol 589:3623-40 (2011). WB ; Mouse . Read more (PubMed: 21606113) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"