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Rabbit polyclonal to EEF2K
Predicted to work with:
Rat, Rabbit, Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Chinese hamster, Orangutan, Elephant
Synthetic peptide, corresponding to a region between amino acids 1-50 of Human EEF2K, NP_037434.1
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
Use at 2-5 µg/mg of lysate.
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Phosphorylates eukaryotic elongation factor-2. Binds calmodulin.
Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
Contains 1 alpha-type protein kinase domain.
Information by UniProt
Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2K antibody (ab85721)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling EEF2K with ab85721 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-EEF2K antibody (ab85721)
All lanes : Anti-EEF2K antibody (ab85721) at 0.1 µg/ml Lane 1 : HeLa cells lysate at 50 µg Lane 2 : HeLa cells lysate at 15 µg Lane 3 : HeLa cells lysate at 5 µg Developed using the ECL technique. Predicted band size: 95 kDa Observed band size: 95 kDa Additional bands at: 110 kDa, 350 kDa. We are unsure as to the identity of these extra bands. Exposure time: 3 minutes
Immunoprecipitation - Anti-EEF2K antibody (ab85721)
Detection of Human EEF2K by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab85721 at 3 ug/mg lysate. Subsequent Western blot detection was performed using 1 ug/ml ab85721.
Detection: Chemiluminescence with exposure times of 30
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"