Overview

  • Product name
    Anti-EEF2K antibody [EP724Y]
    See all EEF2K primary antibodies
  • Description
    Rabbit monoclonal [EP724Y] to EEF2K
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in the Calmodulin binding region of human EEF2K was used as immunogen.

  • Positive control
    • HeLa and Jurkat cell lysates
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab46787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Detects a band of approximately 95 kDa (predicted molecular weight: 82 kDa).
IP 1/20.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      Phosphorylates eukaryotic elongation factor-2. Binds calmodulin.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
      Contains 1 alpha-type protein kinase domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • C86191 antibody
      • Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
      • Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
      • Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase antibody
      • Calmodulin dependent protein kinase III antibody
      • cb365 antibody
      • EC 2.7.11.20 antibody
      • eEF 2 kinase antibody
      • eEF 2K antibody
      • eEF-2 kinase antibody
      • eEF-2K antibody
      • Eef2k antibody
      • EEF2K protein antibody
      • EF2K_HUMAN antibody
      • Elongation factor 2 kinase antibody
      • Eukaryotic elongation factor 2 kinase antibody
      • fa04b08 antibody
      • HSU93850 antibody
      • kinase eEF2K antibody
      • MGC45041 antibody
      • SMEF2K antibody
      • wu:fa04b08 antibody
      • zgc:100973 antibody
      see all

    Images



    • Predicted band size : 82 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: EEF2K knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab46787 observed at 95 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab46787 was shown to specifically react with EEF2K when EEF2K knockout samples were used. Wild-type and EEF2K knockout samples were subjected to SDS-PAGE. ab46787 and ab8245 (loading control to GAPDH) were diluted at 1/50 000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-EEF2K antibody [EP724Y] (ab46787) at 1/50000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 82 kDa
      Observed band size : 95 kDa (why is the actual band size different from the predicted?)

    References

    ab46787 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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