Western blot - Anti-EEF2K antibody [EP724Y] (ab46787)
Predicted band size : 82 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: EEF2K knockout HAP1 cell lysate (20 µg) Lane 3: HeLa cell lysate (20 µg) Lane 4: Jurkat cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab46787 observed at 95 kDa. Red - loading control, ab8245, observed at 37 kDa. ab46787 was shown to specifically react with EEF2K when EEF2K knockout samples were used. Wild-type and EEF2K knockout samples were subjected to SDS-PAGE. ab46787 and ab8245 (loading control to GAPDH) were diluted at 1/50 000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Western blot - EEF2K antibody [EP724Y] (ab46787)
All lanes : Anti-EEF2K antibody [EP724Y] (ab46787) at 1/50000 dilution
Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate