Anti-EEF2K (phospho S359) antibody (ab111511)

Overview

  • Product nameAnti-EEF2K (phospho S359) antibody
    See all EEF2K primary antibodies
  • Description
    Rabbit polyclonal to EEF2K (phospho S359)
  • Specificityab111511 detects endogenous levels of EEF2K only when phosphorylated at serine 359 (Human: Ser359; Mouse: Ser358; Rat: Ser358).
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from Human EEF2K around the phosphorylation site of serine 359 (G-SP-P-R-V).

  • Positive control
    • Human breast carcinoma tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab111511 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionPhosphorylates eukaryotic elongation factor-2. Binds calmodulin.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Alpha-type protein kinase family.
    Contains 1 alpha-type protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • C86191 antibody
    • Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
    • Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
    • Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase antibody
    • Calmodulin dependent protein kinase III antibody
    • cb365 antibody
    • EC 2.7.11.20 antibody
    • eEF 2 kinase antibody
    • eEF 2K antibody
    • eEF-2 kinase antibody
    • eEF-2K antibody
    • Eef2k antibody
    • EEF2K protein antibody
    • EF2K_HUMAN antibody
    • Elongation factor 2 kinase antibody
    • Eukaryotic elongation factor 2 kinase antibody
    • fa04b08 antibody
    • HSU93850 antibody
    • kinase eEF2K antibody
    • MGC45041 antibody
    • SMEF2K antibody
    • wu:fa04b08 antibody
    • zgc:100973 antibody
    see all

Anti-EEF2K (phospho S359) antibody images

  • ab111511, at 1/50 dilution, staining EEF2K in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.

References for Anti-EEF2K (phospho S359) antibody (ab111511)

ab111511 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (colon)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Specification colon
Blocking step background sniper as blocking agent for 8 minute(s) · Concentration: 100%
Fixative formalin
Username

Ms. Cheri Pasch

Verified customer

Submitted Jul 14 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"