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Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer pH: 7.40 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride
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Purity Immunogen affinity purified
Purification notes ab111511 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Function Phosphorylates eukaryotic elongation factor-2. Binds calmodulin.
Sequence similarities Belongs to the protein kinase superfamily. Alpha-type protein kinase family. Contains 1 alpha-type protein kinase domain.
Information by UniProt
Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
Anti-EEF2K (phospho S359) antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EEF2K (phospho S359) antibody (ab111511)
ab111511, at 1/50 dilution, staining EEF2K in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.
References for Anti-EEF2K (phospho S359) antibody (ab111511)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"