Anti-EEF2K (phospho S366) antibody (ab51227)
Key features and details
- Rabbit polyclonal to EEF2K (phospho S366)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EEF2K (phospho S366) antibody
See all EEF2K primary antibodies -
Description
Rabbit polyclonal to EEF2K (phospho S366) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human EEF2K aa 300-400 (phospho S366).
Database link: O00418 -
Positive control
- Hela cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
1/50 - 1/100.
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WB |
1/300 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 95 kDa).
|
Notes |
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IHC-P
1/50 - 1/100. |
WB
1/300 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 95 kDa). |
Target
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Function
Phosphorylates eukaryotic elongation factor-2. Binds calmodulin. -
Sequence similarities
Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
Contains 1 alpha-type protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 29904 Human
- Entrez Gene: 13631 Mouse
- Entrez Gene: 25435 Rat
- Omim: 606968 Human
- SwissProt: O00418 Human
- SwissProt: O08796 Mouse
- SwissProt: P70531 Rat
- Unigene: 498892 Human
see all -
Alternative names
- C86191 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
- Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
see all
Images
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All lanes : Anti-EEF2K (phospho S366) antibody (ab51227) at 1/300 dilution
Lane 1 : Extracts of Hela cells treated with 10% serum for 15mins.
Lane 2 : Extracts of Hela cells treated with 10% serum for 15mins and phosphopeptide.
Predicted band size: 95 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab51227 (right picture is blocked with the phospho peptide)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab51227 has not yet been referenced specifically in any publications.