Anti-EEF2K (phospho S366) antibody (ab51227)


  • Product nameAnti-EEF2K (phospho S366) antibody
    See all EEF2K primary antibodies
  • Description
    Rabbit polyclonal to EEF2K (phospho S366)
  • SpecificityThis antibody detects endogenous levels of EEF2K only when phosphorylated at serine 366.
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized phosphopeptide derived from human EEF2K around the phosphorylation site of serine 366 (T-L-SP-G-S).

  • Positive control
    • Hela cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab51227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/300 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).
ELISA 1/40000.


  • FunctionPhosphorylates eukaryotic elongation factor-2. Binds calmodulin.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Alpha-type protein kinase family.
    Contains 1 alpha-type protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • C86191 antibody
    • Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody
    • Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody
    • Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase antibody
    • Calmodulin dependent protein kinase III antibody
    • cb365 antibody
    • EC antibody
    • eEF 2 kinase antibody
    • eEF 2K antibody
    • eEF-2 kinase antibody
    • eEF-2K antibody
    • Eef2k antibody
    • EEF2K protein antibody
    • EF2K_HUMAN antibody
    • Elongation factor 2 kinase antibody
    • Eukaryotic elongation factor 2 kinase antibody
    • fa04b08 antibody
    • HSU93850 antibody
    • kinase eEF2K antibody
    • MGC45041 antibody
    • SMEF2K antibody
    • wu:fa04b08 antibody
    • zgc:100973 antibody
    see all

Anti-EEF2K (phospho S366) antibody images

  • All lanes : Anti-EEF2K (phospho S366) antibody (ab51227) at 1/300 dilution

    Lane 1 : Extracts of Hela cells treated with 10% serum for 15mins.
    Lane 2 : Extracts of Hela cells treated with 10% serum for 15mins and phosphopeptide.

    Predicted band size : 95 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab51227 (right picture is blocked with the phospho peptide)

References for Anti-EEF2K (phospho S366) antibody (ab51227)

ab51227 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (colon)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Specification colon
Blocking step background sniper as blocking agent for 8 minute(s) · Concentration: 100%
Fixative formalin

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Submitted Jul 14 2015