Overview

  • Product name
    Anti-EFCAB10 antibody
  • Description
    Rabbit polyclonal to EFCAB10
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 67-127 of Human EFCAB10.

  • Positive control
    • Human colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. pH 6.0

Target

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121612 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121612 at 1/75 staining EFCAB10 in Paraffin Embedded Human colon tissue by Immunohistochemistry.
    Note: strong nuclear positivity in glandular cells.

References

ab121612 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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