Overview

  • Product nameAnti-EFCBP2 antibody
    See all EFCBP2 primary antibodies
  • Description
    Mouse polyclonal to EFCBP2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length EFCBP2 protein (Human)

  • Positive control
    • Hela cells and transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab66972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesICC/IF: Use at a concentration of 10 µg/ml.
    Note: ab66972 requires Protein A affinity purification prior to ICC/IF application.
    WB: 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 43 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Tissue specificityExpressed in brain.
    • Sequence similaritiesContains 1 ABM domain.
      Contains 2 EF-hand domains.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • EF hand calcium binding protein 2 antibody
      • EF-hand calcium-binding protein 2 antibody
      • N-terminal EF-hand calcium-binding protein 2 antibody
      • NECA2_HUMAN antibody
      • Necab2 antibody
      • Neuronal calcium binding protein 2 antibody
      • Neuronal calcium-binding protein 2 antibody
      • Stip 2 antibody
      • Stip-2 antibody
      • Stip2 antibody
      • Synaptotagmin interacting protein 2 antibody
      • Synaptotagmin-interacting protein 2 antibody
      see all

    Anti-EFCBP2 antibody images

    • All lanes : Anti-EFCBP2 antibody (ab66972) at 1/500 dilution

      Lane 1 : EFCBP2 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 43 kDa
      Observed band size : 26 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescence of purified ab66972 on HeLa cells. Antibody concentration of 10ug/ml.

    References for Anti-EFCBP2 antibody (ab66972)

    ab66972 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab66972.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"