Anti-EFEMP1/Fibulin-3 antibody (ab106429)
Key features and details
- Rabbit polyclonal to EFEMP1/Fibulin-3
- Suitable for: ICC/IF, WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-EFEMP1/Fibulin-3 antibody
See all EFEMP1/Fibulin-3 primary antibodies -
Description
Rabbit polyclonal to EFEMP1/Fibulin-3 -
Host species
Rabbit -
Specificity
ab106429 is predicted not to cross-react with other Fibulin proteins. -
Tested applications
Suitable for: ICC/IF, WB, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human EFEMP1/Fibulin-3 (C terminal).
Database link: Q12805 -
Positive control
- HeLa cell lysate.
-
General notes
Antibodies should not be exposed to prolonged high temperatures.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Please see notes section. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 20 µg/ml.
|
|
WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 55 kDa.
|
|
ICC |
Use a concentration of 20 µg/ml.
|
Notes |
---|
ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 55 kDa. |
ICC
Use a concentration of 20 µg/ml. |
Target
-
Function
Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth. -
Tissue specificity
In the eye, associated with photoreceptor outer and inner segment regions, the nerve fiber layer, outer nuclear layer and inner and outer plexiform layers of the retina. -
Involvement in disease
Defects in EFEMP1 are a cause of Doyne honeycomb retinal dystrophy (DHRD) [MIM:126600]; also known as malattia leventinese (MLVT) (ML). DHRD is an autosomal dominant disease characterized by yellow-white deposits known as drusen that accumulate beneath the retinal pigment epithelium. -
Sequence similarities
Belongs to the fibulin family.
Contains 6 EGF-like domains. -
Cellular localization
Secreted > extracellular space. Secreted > extracellular space > extracellular matrix. Localizes to the lamina propria underneath the olfactory epithelium. - Information by UniProt
-
Database links
- Entrez Gene: 2202 Human
- Omim: 601548 Human
- SwissProt: Q12805 Human
- Unigene: 76224 Human
-
Alternative names
- DHRD antibody
- Doyne honeycomb retinal degeneration antibody
- DRAD antibody
see all
Images
-
Lane 1 : Anti-EFEMP1/Fibulin-3 antibody (ab106429) at 0.5 µg/ml
Lane 2 : Anti-EFEMP1/Fibulin-3 antibody (ab106429) at 1 µg/ml
All lanes : HeLa cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 55 kDa -
Immunohistochemical analysis of HeLa cells labelling EFEMP1/Fibulin-3 with ab106429 at 20 µg/ml.
-
Immunofluorescence analysis of Hela cells labelling Fibulin 3 with ab106429 at 20 ug/ml.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab106429 has been referenced in 3 publications.
- Takahashi R et al. Defining Transcriptional Signatures of Human Hair Follicle Cell States. J Invest Dermatol 140:764-773.e4 (2020). PubMed: 31676413
- Zhang N et al. Hypermethylation of EFEMP1 in the Hippocampus May Be Related to the Deficit in Spatial Memory of Rat Neonates Triggered by Repeated Administration of Propofol. Biomed Res Int 2020:8851480 (2020). PubMed: 33381589
- Shao L et al. Genomic expression profiles in cumulus cells derived from germinal vesicle and MII mouse oocytes. Reprod Fertil Dev N/A:N/A (2015). PubMed: 25989843