Anti-EFEMP1/Fibulin-3 antibody (ab151976)
Key features and details
- Rabbit polyclonal to EFEMP1/Fibulin-3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-EFEMP1/Fibulin-3 antibody
See all EFEMP1/Fibulin-3 primary antibodies -
Description
Rabbit polyclonal to EFEMP1/Fibulin-3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human EFEMP1/Fibulin-3 aa 168-488.
Database link: Q12805 -
Positive control
- WB: HEK-293T, A431, H1299, HepG2 and HeLa whole cell lysates; rat liver tissue lysate; mouse liver tissue lysate. IHC-P: NCIN87 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 54 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 54 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth. -
Tissue specificity
In the eye, associated with photoreceptor outer and inner segment regions, the nerve fiber layer, outer nuclear layer and inner and outer plexiform layers of the retina. -
Involvement in disease
Defects in EFEMP1 are a cause of Doyne honeycomb retinal dystrophy (DHRD) [MIM:126600]; also known as malattia leventinese (MLVT) (ML). DHRD is an autosomal dominant disease characterized by yellow-white deposits known as drusen that accumulate beneath the retinal pigment epithelium. -
Sequence similarities
Belongs to the fibulin family.
Contains 6 EGF-like domains. -
Cellular localization
Secreted > extracellular space. Secreted > extracellular space > extracellular matrix. Localizes to the lamina propria underneath the olfactory epithelium. - Information by UniProt
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Database links
- Entrez Gene: 2202 Human
- Entrez Gene: 216616 Mouse
- Entrez Gene: 305604 Rat
- Omim: 601548 Human
- SwissProt: Q12805 Human
- SwissProt: Q8BPB5 Mouse
- SwissProt: O35568 Rat
- Unigene: 76224 Human
see all -
Alternative names
- DHRD antibody
- Doyne honeycomb retinal degeneration antibody
- DRAD antibody
see all
Images
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All lanes : Anti-EFEMP1/Fibulin-3 antibody (ab151976) at 1/1000 dilution
Lane 1 : HEK-293T whole cell extracts
Lane 2 : A431 whole cell extracts
Lane 3 : HeLa whole cell extracts
Lane 4 : HepG2 whole cell extracts
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa10% SDS-PAGE
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All lanes : Anti-EFEMP1/Fibulin-3 antibody (ab151976) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T whole cell extracts
Lane 2 : Transfected HEK-293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa10% SDS-PAGE
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Anti-EFEMP1/Fibulin-3 antibody (ab151976) at 1/500 dilution + rat liver lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 54 kDa -
Anti-EFEMP1/Fibulin-3 antibody (ab151976) at 1/1000 dilution + Mouse liver lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 54 kDa -
Immunohistochemical analysis of paraffin embedded NCIN87 xenograft tissue labeling EFEMP1/Fibulin-3 with ab151976 at a 1/500 dilution.
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All lanes : Anti-EFEMP1/Fibulin-3 antibody (ab151976) at 1/1000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa
10% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab151976 has been referenced in 1 publication.
- Chen Y et al. A genome-wide association study identifies a susceptibility locus for biliary atresia on 2p16.1 within the gene EFEMP1. PLoS Genet 14:e1007532 (2018). PubMed: 30102696