Recombinant
RabMAb

Anti-EFEMP2 antibody [EPR684(2)] (ab125073)

Overview

  • Product name
    Anti-EFEMP2 antibody [EPR684(2)]
    See all EFEMP2 primary antibodies
  • Description
    Rabbit monoclonal [EPR684(2)] to EFEMP2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human EFEMP2.

  • Positive control
    • JAR and SW480 cell lysates, Human fetal lung lysate, Human breast carcinoma and muscle tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab125073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Involvement in disease
      Defects in EFEMP2 are a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100]. Hereditary cutis laxa refers to a heterogeneous group of connective tissue disorders characterized by cutaneous abnormalities and variable systemic manifestations. The most constant clinical feature is loose skin, sagging over the face and trunk. Hereditary cutis laxa is inherited in both autosomal dominant and autosomal recessive modes. ARCL1 shows the most severe phenotype and has the poorest prognosis. In addition to the skin, internal organs enriched in elastic fibers, such as the lung and arteries, are affected.
    • Sequence similarities
      Belongs to the fibulin family.
      Contains 6 EGF-like domains.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • EFEMP 2 antibody
      • EFEMP2 antibody
      • EGF containing fibulin like extracellular matrix protein 2 antibody
      • EGF-containing fibulin-like extracellular matrix protein 2 antibody
      • FBLN 4 antibody
      • FBLN4 antibody
      • FBLN4_HUMAN antibody
      • FIBL 4 antibody
      • FIBL-4 antibody
      • FIBL4 antibody
      • Fibulin 4 antibody
      • Fibulin-4 antibody
      • MBP 1 antibody
      • MBP1 antibody
      • Mutant p53 binding protein 1 antibody
      • Protein UPH1 antibody
      • UPH 1 antibody
      • UPH1 antibody
      • UPH1 protein antibody
      see all

    Images

    • All lanes : Anti-EFEMP2 antibody [EPR684(2)] (ab125073) at 1/1000 dilution

      Lane 1 : HEK-293T (human embryonic kidney) whole cell lysates
      Lane 2 : HepG2 (human hepatocellular carcinoma) whole cell lysates
      Lane 3 : Human fetal lung lysates
      Lane 4 : Human colon cancer lysates
      Lane 5 : Human placenta lysates
      Lane 6 : Mouse placenta lysates
      Lane 7 : Moue lung lysates
      Lane 8 : NIH/3T3 (mouse embryo) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 49 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-EFEMP2 antibody [EPR684(2)] (ab125073) at 1/1000 dilution

      Lane 1 : JAR cell lysate
      Lane 2 : SW480 cell lysate
      Lane 3 : Human fetal lung lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 49 kDa
    • ab125073, at 1/250, staining EFEMP2 in paraffin-embedded Human breast carcinoma tissue by immunohistochemistry.
    • ab125073, at 1/250, staining EFEMP2 in paraffin-embedded Human muscle tissue by immunohistochemistry.

    References

    This product has been referenced in:
    • Wang T  et al. Fibulin-4 is associated with prognosis of endometrial cancer patients and inhibits cancer cell invasion and metastasis via Wnt/ß-catenin signaling pathway. Oncotarget 8:18991-19012 (2017). ICC ; Human . Read more (PubMed: 28177909) »
    • Zhang D  et al. Fibulin-4 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition via the PI3K/Akt/mTOR pathway. Int J Oncol 50:1513-1530 (2017). WB, IHC-P, ICC/IF ; Human . Read more (PubMed: 28339091) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer (10mM Sodium Citrate, 0.05% Tween 20, pH 6.0)
    Sample
    Human Tissue sections (human colon - smooth muscle cells)
    Specification
    human colon - smooth muscle cells
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Mrs. Maria Cecilia Ybanez

    Verified customer

    Submitted Jan 23 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer (10mM Sodium Citrate, 0.05% T ween 20, pH 6.0)
    Sample
    Human Tissue sections (human pancreas)
    Specification
    human pancreas
    Permeabilization
    Yes - 0.2% Triton X-100 and PBS
    Fixative
    Formaldehyde
    Username

    Dr. Steffen Rickelt

    Verified customer

    Submitted Nov 06 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up