Overview

  • Product name
    Anti-EFHA1 antibody
  • Description
    Rabbit polyclonal to EFHA1
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 95-238 of Human EFHA1 (AAH31089).

  • Positive control
    • U87-MG whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.

Target

Images

  • Anti-EFHA1 antibody (ab101465) at 1/1000 dilution + U87-MG whole cell lysate at 30 µg

    Predicted band size : 50 kDa
    10% SDS-PAGE
  • Immunohistochemical analysis of FFPE mouse kidney tissue, labeling EFHA1 with ab101465 (diluted 1/500).

  • Immunohistochemical analysis of FFPE human hepatoma tissue, labeling EFHA1 with ab101465 (diluted 1/500).

  • Immunoflourescent analysis of methanol-fixed MCF-7 cell line, labeling EFHA1 with ab101465 (diluted 1/200). DNA stained with Hoechst 33342.

References

This product has been referenced in:
  • Vais H  et al. EMRE Is a Matrix Ca(2+) Sensor that Governs Gatekeeping of the Mitochondrial Ca(2+) Uniporter. Cell Rep 14:403-410 (2016). Read more (PubMed: 26774479) »
  • Lee Y  et al. Structure and function of the N-terminal domain of the human mitochondrial calcium uniporter. EMBO Rep N/A:N/A (2015). WB ; Human . Read more (PubMed: 26341627) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
25 µg
Specification
Prostate cancer cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 04 2016

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (4-12%)
Sample
Mouse Tissue lysate - whole (heart)
Specification
heart
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. Giulia Borile

Verified customer

Submitted Jun 23 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (4-12% BIS-TRIS)
Sample
Human Cell lysate - whole cell (fibroblasts)
Specification
fibroblasts
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Feb 20 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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