Overview

  • Product nameAnti-EFHA1 antibody
    See all EFHA1 primary antibodies
  • Description
    Rabbit polyclonal to EFHA1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 95-238 of Human EFHA1 (AAH31089).

  • Positive control
    • U87-MG whole cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.

Target

Anti-EFHA1 antibody images

  • Anti-EFHA1 antibody (ab101465) at 1/1000 dilution + U87-MG whole cell lysate at 30 µg

    Predicted band size : 50 kDa

References for Anti-EFHA1 antibody (ab101465)

This product has been referenced in:
  • Lee Y  et al. Structure and function of the N-terminal domain of the human mitochondrial calcium uniporter. EMBO Rep N/A:N/A (2015). WB ; Human . Read more (PubMed: 26341627) »
  • Plovanich M  et al. MICU2, a paralog of MICU1, resides within the mitochondrial uniporter complex to regulate calcium handling. PLoS One 8:e55785 (2013). WB ; Human . Read more (PubMed: 23409044) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Prostate cancer cell line)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 25 µg
Specification Prostate cancer cell line
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 04 2016

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (4-12%)
Sample Mouse Tissue lysate - whole (heart)
Specification heart
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. Giulia Borile

Verified customer

Submitted Jun 23 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (4-12% BIS-TRIS)
Sample Human Cell lysate - whole cell (fibroblasts)
Specification fibroblasts
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Feb 20 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"