Overview

  • Product nameAnti-EFHC1 antibody
  • Description
    Rabbit polyclonal to EFHC1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Mouse EFHC1.

    (Peptide available as ab93070.)

  • Positive control
    • This antibody gave a positive signal in the following mouse tissue lysates: Hippocampus; Cortex.

Properties

Applications

Our Abpromise guarantee covers the use of ab80861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 75 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceEFHC1 gene encodes a protein with an EF-hand motif; EFHC1 protein affects programmed cell death through effects on calcium influx; overexpression of EFHC1 in hippocampal primary culture neurons induces apoptosis. EFHC1 is widely expressed.
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • EF hand antibody
      • EF hand domain containing protein 1 antibody
      • Myoclonin 1 antibody
      • Myoclonin-1 antibody

    Anti-EFHC1 antibody images

    • All lanes : Anti-EFHC1 antibody (ab80861) at 1 µg/ml

      Lane 1 : Mouse Cortex Tissue Lysate
      Lane 2 : Mouse Hippocampus Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 75 kDa
      Observed band size : 77 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 150 kDa,56 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 10 minutes

    References for Anti-EFHC1 antibody (ab80861)

    ab80861 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Human lung cell line)
    Permeabilization Yes - 0.5% Triton X100 in PBS
    Specification Human lung cell line
    Blocking step 3%BSA+5%Normal Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative Methanol
    Username

    Abcam user community

    Verified customer

    Submitted Oct 12 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (P5 and adult mouse cerebellum)
    Specification P5 and adult mouse cerebellum
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid HIER
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Sep 06 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Adult Cerebellum)
    Specification Adult Cerebellum
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization No
    Blocking step (agent) for 10 minute(s) · Concentration: 1% · Temperature: 20°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Aug 20 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"