Overview

  • Product nameAnti-EFHD2 antibody
    See all EFHD2 primary antibodies
  • Description
    Goat polyclonal to EFHD2
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human EFHD2 aa 230-240 (C terminal).
    Sequence:

    (C)AAFKELQSTFK

  • Positive control
    • WB: Human tonsil, human thymus, human lymph node and mouse thymus tissue lysates. IHC-P: Human tonsil tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 28-30 kDa.

Target

  • FunctionMay regulate B-cell receptor (BCR)-induced immature and primary B-cell apoptosis (By similarity). Plays a role as negative regulator of the canonical NF-kappa-B-activating branch (By similarity). Controls spontaneous apoptosis through the regulation of BCL2L1 abundance.
  • Tissue specificityFound in lymphocytes; preferentially expressed in CD8+ cells.
  • Sequence similaritiesContains 2 EF-hand domains.
  • Cellular localizationMembrane raft. In immature B-cell line WEHI231.
  • Information by UniProt
  • Database links
  • Alternative names
    • EF hand domain containing protein 2 antibody
    • EF hand domain family, member D2 antibody
    • EF-hand domain-containing protein D2 antibody
    • EFHD 2 antibody
    • EFHD2 antibody
    • EFHD2_HUMAN antibody
    • RP3-467K16.3 antibody
    • Swiprosin 1 antibody
    • Swiprosin-1 antibody
    • SWS1 antibody
    see all

Anti-EFHD2 antibody images

  • All lanes : Anti-EFHD2 antibody (ab24368) at 0.3 µg/ml

    Lane 1 : Human tonsil lysate
    Lane 2 : Human thymus lysate
    Lane 3 : Human lymph node lysate
    Lane 4 : Mouse thymus lysate

    Lysates/proteins at 35 µg per lane.
  • Immunohistochemistry (Paraffin-embedded sections) analysis of paraffin embedded human tonsil tissue labeling EFHD2 with ab24368 at 2 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

  • ab24368 staining EFHD2 in Human neuroblastoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS + 5% serum) for 1 hour. DyLight® 594-conjugated Mouse anti-goat IgG polyclonal (7µg/ml) was used as the secondary antibody.

    See Abreview

References for Anti-EFHD2 antibody (ab24368)

This product has been referenced in:
  • Borger E  et al. The calcium-binding protein EFhd2 modulates synapse formation in vitro and is linked to human dementia. J Neuropathol Exp Neurol 73:1166-82 (2014). WB . Read more (PubMed: 25383639) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (Neuroblastoma)
Specification Neuroblastoma
Permeabilization Yes - 0.1% Tx100, 5min
Fixative Formaldehyde
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Submitted May 28 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"