The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 28-30 kDa.
FunctionMay regulate B-cell receptor (BCR)-induced immature and primary B-cell apoptosis (By similarity). Plays a role as negative regulator of the canonical NF-kappa-B-activating branch (By similarity). Controls spontaneous apoptosis through the regulation of BCL2L1 abundance.
Tissue specificityFound in lymphocytes; preferentially expressed in CD8+ cells.
Sequence similaritiesContains 2 EF-hand domains.
Cellular localizationMembrane raft. In immature B-cell line WEHI231.
Immunohistochemistry (Paraffin-embedded sections) analysis of paraffin embedded human tonsil tissue labeling EFHD2 with ab24368 at 2 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunocytochemistry/ Immunofluorescence - Anti-EFHD2 antibody (ab24368)This image is courtesy of an anonymous Abreview
ab24368 staining EFHD2 in Human neuroblastoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS + 5% serum) for 1 hour. DyLight® 594-conjugated Mouse anti-goat IgG polyclonal (7µg/ml) was used as the secondary antibody.